Longitudinal IgA and IgG Response, and ACE2 Binding Blockade, to Full-Length SARS-CoV-2 Spike Protein Variants in a Population of Black PLWH Vaccinated with ChAdOx1 nCoV-19
Vaccines against SARS-CoV-2 have been pivotal in overcoming the COVID-19 pandemic yet understanding the subsequent outcomes and immunological effects remain crucial, especially for at-risk groups e.g., people living with human immunodeficiency virus (HIV) (PLWH). In this study we report the longitudinal IgA and IgG antibody titers, as well as antibody-mediated angiotensin converting enzyme 2 (ACE2) binding blockade, against the SARS-CoV-2 spike (S) proteins after 1 and 2 doses of the ChAdOx1 nCoV-19 vaccine in a population of Black PLWH. Here, we report that PLWH (N = 103) did not produce an anti-S IgA response after infection or vaccination, however, anti-S IgG was detected in response to vaccination and infection, with the highest level detected for infected vaccinated participants. The anti-IgG and ACE2 blockade assays revealed that both vaccination and infection resulted in IgG production, however, only vaccination resulted in a moderate increase in ACE2 binding blockade to the ancestral S protein. Vaccination with a previous infection results in the greatest anti-S IgG and ACE2 blockade for the ancestral S protein. In conclusion, PLWH produce an anti-S IgG response to the ChAdOx1 nCoV-19 vaccine and/or infection, and ChAdOx1 nCoV-19 vaccination with a previous infection produced more neutralizing antibodies than vaccination alone.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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| Enthalten in: |
Viruses - 15(2023), 2 vom: 06. Feb. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Smith, Muneerah [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 07.03.2023 Date Revised 10.04.2023 published: Electronic ClinicalTrials.gov: NCT04444674 PACTR: ACTR202006922165132 Citation Status MEDLINE |
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| doi: |
10.3390/v15020448 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM353577464 |
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| 100 | 1 | |a Smith, Muneerah |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Longitudinal IgA and IgG Response, and ACE2 Binding Blockade, to Full-Length SARS-CoV-2 Spike Protein Variants in a Population of Black PLWH Vaccinated with ChAdOx1 nCoV-19 |
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| 500 | |a Date Completed 07.03.2023 | ||
| 500 | |a Date Revised 10.04.2023 | ||
| 500 | |a published: Electronic | ||
| 500 | |a ClinicalTrials.gov: NCT04444674 | ||
| 500 | |a PACTR: ACTR202006922165132 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Vaccines against SARS-CoV-2 have been pivotal in overcoming the COVID-19 pandemic yet understanding the subsequent outcomes and immunological effects remain crucial, especially for at-risk groups e.g., people living with human immunodeficiency virus (HIV) (PLWH). In this study we report the longitudinal IgA and IgG antibody titers, as well as antibody-mediated angiotensin converting enzyme 2 (ACE2) binding blockade, against the SARS-CoV-2 spike (S) proteins after 1 and 2 doses of the ChAdOx1 nCoV-19 vaccine in a population of Black PLWH. Here, we report that PLWH (N = 103) did not produce an anti-S IgA response after infection or vaccination, however, anti-S IgG was detected in response to vaccination and infection, with the highest level detected for infected vaccinated participants. The anti-IgG and ACE2 blockade assays revealed that both vaccination and infection resulted in IgG production, however, only vaccination resulted in a moderate increase in ACE2 binding blockade to the ancestral S protein. Vaccination with a previous infection results in the greatest anti-S IgG and ACE2 blockade for the ancestral S protein. In conclusion, PLWH produce an anti-S IgG response to the ChAdOx1 nCoV-19 vaccine and/or infection, and ChAdOx1 nCoV-19 vaccination with a previous infection produced more neutralizing antibodies than vaccination alone | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
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| 700 | 1 | |a Kwatra, Gaurav |e verfasserin |4 aut | |
| 700 | 1 | |a Izu, Alane |e verfasserin |4 aut | |
| 700 | 1 | |a Nel, Andrew |e verfasserin |4 aut | |
| 700 | 1 | |a Cutland, Clare |e verfasserin |4 aut | |
| 700 | 1 | |a Ahmed, Khatja |e verfasserin |4 aut | |
| 700 | 1 | |a Baillie, Vicky |e verfasserin |4 aut | |
| 700 | 1 | |a Barnabas, Shaun |e verfasserin |4 aut | |
| 700 | 1 | |a Bhorat, Qasim |e verfasserin |4 aut | |
| 700 | 1 | |a Briner, Carmen |e verfasserin |4 aut | |
| 700 | 1 | |a Lazarus, Erica |e verfasserin |4 aut | |
| 700 | 1 | |a Dheda, Keertan |e verfasserin |4 aut | |
| 700 | 1 | |a Fairlie, Lee |e verfasserin |4 aut | |
| 700 | 1 | |a Koen, Anthonet |e verfasserin |4 aut | |
| 700 | 1 | |a Madhi, Shabir |e verfasserin |4 aut | |
| 700 | 1 | |a Blackburn, Jonathan M |e verfasserin |4 aut | |
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