Modulation of the Aryl Hydrocarbon Receptor Signaling Pathway Impacts on Junín Virus Replication
Junín virus (JUNV), a member of the family Arenaviridae, is the etiological agent of the Argentine hemorrhagic fever, an endemic disease in the rural region of Argentina lacking a specific chemotherapy. Aryl hydrocarbon receptor (AHR) is expressed in several mammalian tissues and has been indicated as a sensor of ligands from variable sources and a modulator of the cell immune response. Interestingly, recent studies have suggested that the activation or depression of the AHR signaling pathway may play a role in the outcome of diverse human viral infections. In the present report, the effect of the pharmacological modulation of AHR on JUNV in vitro infection was analyzed. An initial microarray screening showed that the AHR pathway was overexpressed in JUNV-infected hepatic cells. Concomitantly, the infection of Vero and Huh-7 cells with the JUNV strains IV4454 and Candid#1 was significantly inhibited in a dose-dependent manner by treatment with CH223191, a specific AHR antagonist, as detected by infectivity assays, real-time RT-PCR and immunofluorescence detection of viral proteins. Furthermore, the pro-viral role of AHR in JUNV infection appears to be independent of the IFN-I pathway. Our findings support the promising perspectives of the pharmacological modulation of AHR as a potential target for the control of AHF.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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| Enthalten in: |
Viruses - 15(2023), 2 vom: 28. Jan. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Pelaez, Miguel Angel [VerfasserIn] |
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| Links: |
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| Themen: |
Arenavirus |
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| Anmerkungen: |
Date Completed 06.03.2023 Date Revised 11.04.2023 published: Electronic Citation Status MEDLINE |
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| doi: |
10.3390/v15020369 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM353576670 |
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| 100 | 1 | |a Pelaez, Miguel Angel |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Modulation of the Aryl Hydrocarbon Receptor Signaling Pathway Impacts on Junín Virus Replication |
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| 500 | |a Date Revised 11.04.2023 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Junín virus (JUNV), a member of the family Arenaviridae, is the etiological agent of the Argentine hemorrhagic fever, an endemic disease in the rural region of Argentina lacking a specific chemotherapy. Aryl hydrocarbon receptor (AHR) is expressed in several mammalian tissues and has been indicated as a sensor of ligands from variable sources and a modulator of the cell immune response. Interestingly, recent studies have suggested that the activation or depression of the AHR signaling pathway may play a role in the outcome of diverse human viral infections. In the present report, the effect of the pharmacological modulation of AHR on JUNV in vitro infection was analyzed. An initial microarray screening showed that the AHR pathway was overexpressed in JUNV-infected hepatic cells. Concomitantly, the infection of Vero and Huh-7 cells with the JUNV strains IV4454 and Candid#1 was significantly inhibited in a dose-dependent manner by treatment with CH223191, a specific AHR antagonist, as detected by infectivity assays, real-time RT-PCR and immunofluorescence detection of viral proteins. Furthermore, the pro-viral role of AHR in JUNV infection appears to be independent of the IFN-I pathway. Our findings support the promising perspectives of the pharmacological modulation of AHR as a potential target for the control of AHF | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Argentine hemorrhagic fever | |
| 650 | 4 | |a Junín virus | |
| 650 | 4 | |a arenavirus | |
| 650 | 4 | |a aryl hydrocarbon receptor | |
| 650 | 4 | |a host therapeutic target | |
| 650 | 7 | |a Receptors, Aryl Hydrocarbon |2 NLM | |
| 700 | 1 | |a Torti, María Florencia |e verfasserin |4 aut | |
| 700 | 1 | |a Alvarez De Lauro, Aaron Ezequiel |e verfasserin |4 aut | |
| 700 | 1 | |a Marquez, Agostina Belén |e verfasserin |4 aut | |
| 700 | 1 | |a Giovannoni, Federico |e verfasserin |4 aut | |
| 700 | 1 | |a Damonte, Elsa Beatriz |e verfasserin |4 aut | |
| 700 | 1 | |a García, Cybele Carina |e verfasserin |4 aut | |
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| 856 | 4 | 0 | |u http://dx.doi.org/10.3390/v15020369 |3 Volltext |
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