Biosynthesis and Properties of Sulfur-Containing Polyhydroxyalkanoates (PHAs) Produced by Wild-Type Strain Cupriavidus necator B-10646
The study addresses the growth of the wild-type strain Cupriavidus necator B-10646 and the synthesis of sulfur-containing polyhydroxyalkanoates (PHA) by this strain on media containing fructose and three different precursors (3-mercaptopropionic acid, 3',3'-dithiodipropionic acid and 3',3'-thiodipropionic acid). By varying the concentration and number of doses of the precursors added into the bacterial culture, it was possible to find conditions that ensure the formation of 3-mercaptopropionate (3MP) monomers from the precursors and their incorporation into the C-chain of poly(3-hydroxybutyrate). A series of P(3HB-co-3MP) copolymer samples with different content of 3MP monomers (from 2.04 to 39.0 mol.%) were synthesized and the physicochemical properties were studied. The effect of 3MP monomers is manifested in a certain decrease in the molecular weight of the samples and an increase in polydispersity. Temperature changes are manifested in the appearance of two peaks in the melting region with different intervals regardless of the 3MP content. The studied P(3HB-co-3MP) samples, regardless of the content of 3MP monomers, are characterized by equalization of the ratio of the amorphous and crystalline phases and have a close degree of crystallinity with a minimum of 42%, = and a maximum of 54%.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2023 |
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Erschienen: |
2023 |
Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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Enthalten in: |
Polymers - 15(2023), 4 vom: 17. Feb. |
Sprache: |
Englisch |
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Beteiligte Personen: |
Zhila, Natalia O [VerfasserIn] |
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Links: |
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Themen: |
3MP monomers contents |
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Anmerkungen: |
Date Revised 03.03.2023 published: Electronic Citation Status PubMed-not-MEDLINE |
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doi: |
10.3390/polym15041005 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM353563757 |
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520 | |a The study addresses the growth of the wild-type strain Cupriavidus necator B-10646 and the synthesis of sulfur-containing polyhydroxyalkanoates (PHA) by this strain on media containing fructose and three different precursors (3-mercaptopropionic acid, 3',3'-dithiodipropionic acid and 3',3'-thiodipropionic acid). By varying the concentration and number of doses of the precursors added into the bacterial culture, it was possible to find conditions that ensure the formation of 3-mercaptopropionate (3MP) monomers from the precursors and their incorporation into the C-chain of poly(3-hydroxybutyrate). A series of P(3HB-co-3MP) copolymer samples with different content of 3MP monomers (from 2.04 to 39.0 mol.%) were synthesized and the physicochemical properties were studied. The effect of 3MP monomers is manifested in a certain decrease in the molecular weight of the samples and an increase in polydispersity. Temperature changes are manifested in the appearance of two peaks in the melting region with different intervals regardless of the 3MP content. The studied P(3HB-co-3MP) samples, regardless of the content of 3MP monomers, are characterized by equalization of the ratio of the amorphous and crystalline phases and have a close degree of crystallinity with a minimum of 42%, = and a maximum of 54% | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a 3MP monomers contents | |
650 | 4 | |a biosynthesis | |
650 | 4 | |a chemical composition | |
650 | 4 | |a different precursors | |
650 | 4 | |a properties | |
650 | 4 | |a sulfur-containing PHAs | |
700 | 1 | |a Sapozhnikova, Kristina Yu |e verfasserin |4 aut | |
700 | 1 | |a Berezovskaya, Arina V |e verfasserin |4 aut | |
700 | 1 | |a Kiselev, Evgeniy G |e verfasserin |4 aut | |
700 | 1 | |a Shishatskaya, Ekaterina I |e verfasserin |4 aut | |
700 | 1 | |a Vasiliev, Aleksander D |e verfasserin |4 aut | |
700 | 1 | |a Thomas, Sabu |e verfasserin |4 aut | |
700 | 1 | |a Volova, Tatiana G |e verfasserin |4 aut | |
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