Mechanism of Aspirin oxidative stress regulating interleukin-induced apoptosis in nucleus pulposus cells in a rat model of intervertebral disc degeneration
2023 Annals of Translational Medicine. All rights reserved..
Background: Intervertebral disc degeneration (IDD) is an important cause of low back pain. Increase of reactive oxygen species (ROS), overexpression of inflammatory factors, and loss of extracellular matrix are important factors in the pathological changes of IDD. The present study aimed to investigate the mechanism of action of Aspirin regulating oxidative stress in IDD, so as to propose new treatment.
Methods: Nucleus pulposus cells (NPCs) were isolated from the caudal intervertebral discs of Sprague Dawley (SD) rats under sterile conditions. The expression of ROS and inflammatory factors was detected sequentially, and the degree of degeneration of nucleus pulposus cells was observed by real-time fluorescence quantitative polymerase chain reaction (PCR) and cell immunofluorescence staining. In vivo, the caudal disc puncture model was used to induce degeneration, and a local injection of 10 or 100 µg/mL Aspirin was performed. The rats were sacrificed 1 week later, and the disc specimens of the tail vertebrae were collected for imaging, histomorphology, and immunohistochemical analysis.
Results: In vitro experiments showed that lipopolysaccharide (LPS) could significantly induce oxidative stress in NPCs and stimulate NPCs to secrete a large amount of ROS and inflammatory factors, which eventually leads to the reduction of collagen type II and polyglycoprotein gene expression in NPCs and the high expression of matrix metalloproteinase (MMP). Consequently, NPCs degeneration occurs.
Conclusions: Our results clarified the important role of oxidative stress in IDD and proved that LPS can be used as a drug to alleviate oxidative stress and intervene in the IDD process.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
Annals of translational medicine - 11(2023), 2 vom: 31. Jan., Seite 124 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liang, He [VerfasserIn] |
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| Links: |
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| Themen: |
Adenosine monophosphate-activated protein kinase (AMPK) |
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| Anmerkungen: |
Date Revised 24.02.2023 published: Print Citation Status PubMed-not-MEDLINE |
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| doi: |
10.21037/atm-22-5700 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM353257435 |
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| 245 | 1 | 0 | |a Mechanism of Aspirin oxidative stress regulating interleukin-induced apoptosis in nucleus pulposus cells in a rat model of intervertebral disc degeneration |
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| 500 | |a Date Revised 24.02.2023 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a 2023 Annals of Translational Medicine. All rights reserved. | ||
| 520 | |a Background: Intervertebral disc degeneration (IDD) is an important cause of low back pain. Increase of reactive oxygen species (ROS), overexpression of inflammatory factors, and loss of extracellular matrix are important factors in the pathological changes of IDD. The present study aimed to investigate the mechanism of action of Aspirin regulating oxidative stress in IDD, so as to propose new treatment | ||
| 520 | |a Methods: Nucleus pulposus cells (NPCs) were isolated from the caudal intervertebral discs of Sprague Dawley (SD) rats under sterile conditions. The expression of ROS and inflammatory factors was detected sequentially, and the degree of degeneration of nucleus pulposus cells was observed by real-time fluorescence quantitative polymerase chain reaction (PCR) and cell immunofluorescence staining. In vivo, the caudal disc puncture model was used to induce degeneration, and a local injection of 10 or 100 µg/mL Aspirin was performed. The rats were sacrificed 1 week later, and the disc specimens of the tail vertebrae were collected for imaging, histomorphology, and immunohistochemical analysis | ||
| 520 | |a Results: In vitro experiments showed that lipopolysaccharide (LPS) could significantly induce oxidative stress in NPCs and stimulate NPCs to secrete a large amount of ROS and inflammatory factors, which eventually leads to the reduction of collagen type II and polyglycoprotein gene expression in NPCs and the high expression of matrix metalloproteinase (MMP). Consequently, NPCs degeneration occurs | ||
| 520 | |a Conclusions: Our results clarified the important role of oxidative stress in IDD and proved that LPS can be used as a drug to alleviate oxidative stress and intervene in the IDD process | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Aspirin | |
| 650 | 4 | |a adenosine monophosphate-activated protein kinase (AMPK) | |
| 650 | 4 | |a intervertebral disc degeneration (IDD) | |
| 650 | 4 | |a nucleus pulposus cells (NPCs) | |
| 650 | 4 | |a oxidative stress | |
| 700 | 1 | |a Shi, Hongyang |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Yang |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Deguo |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yajie |e verfasserin |4 aut | |
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| 773 | 1 | 8 | |g volume:11 |g year:2023 |g number:2 |g day:31 |g month:01 |g pages:124 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.21037/atm-22-5700 |3 Volltext |
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