High cell density and high-resolution 3D bioprinting for fabricating vascularized tissues
Three-dimensional (3D) bioprinting techniques have emerged as the most popular methods to fabricate 3D-engineered tissues; however, there are challenges in simultaneously satisfying the requirements of high cell density (HCD), high cell viability, and fine fabrication resolution. In particular, bioprinting resolution of digital light processing-based 3D bioprinting suffers with increasing bioink cell density due to light scattering. We developed a novel approach to mitigate this scattering-induced deterioration of bioprinting resolution. The inclusion of iodixanol in the bioink enables a 10-fold reduction in light scattering and a substantial improvement in fabrication resolution for bioinks with an HCD. Fifty-micrometer fabrication resolution was achieved for a bioink with 0.1 billion per milliliter cell density. To showcase the potential application in tissue/organ 3D bioprinting, HCD thick tissues with fine vascular networks were fabricated. The tissues were viable in a perfusion culture system, with endothelialization and angiogenesis observed after 14 days of culture.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
|---|---|
| Enthalten in: |
Science advances - 9(2023), 8 vom: 22. Feb., Seite eade7923 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
You, Shangting [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
|---|
| Anmerkungen: |
Date Completed 24.02.2023 Date Revised 29.12.2023 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1126/sciadv.ade7923 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM353185973 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM353185973 | ||
| 003 | DE-627 | ||
| 005 | 20240108135849.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1126/sciadv.ade7923 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1242.xml |
| 035 | |a (DE-627)NLM353185973 | ||
| 035 | |a (NLM)36812321 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a You, Shangting |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a High cell density and high-resolution 3D bioprinting for fabricating vascularized tissues |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 24.02.2023 | ||
| 500 | |a Date Revised 29.12.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Three-dimensional (3D) bioprinting techniques have emerged as the most popular methods to fabricate 3D-engineered tissues; however, there are challenges in simultaneously satisfying the requirements of high cell density (HCD), high cell viability, and fine fabrication resolution. In particular, bioprinting resolution of digital light processing-based 3D bioprinting suffers with increasing bioink cell density due to light scattering. We developed a novel approach to mitigate this scattering-induced deterioration of bioprinting resolution. The inclusion of iodixanol in the bioink enables a 10-fold reduction in light scattering and a substantial improvement in fabrication resolution for bioinks with an HCD. Fifty-micrometer fabrication resolution was achieved for a bioink with 0.1 billion per milliliter cell density. To showcase the potential application in tissue/organ 3D bioprinting, HCD thick tissues with fine vascular networks were fabricated. The tissues were viable in a perfusion culture system, with endothelialization and angiogenesis observed after 14 days of culture | ||
| 650 | 4 | |a Journal Article | |
| 700 | 1 | |a Xiang, Yi |e verfasserin |4 aut | |
| 700 | 1 | |a Hwang, Henry H |e verfasserin |4 aut | |
| 700 | 1 | |a Berry, David B |e verfasserin |4 aut | |
| 700 | 1 | |a Kiratitanaporn, Wisarut |e verfasserin |4 aut | |
| 700 | 1 | |a Guan, Jiaao |e verfasserin |4 aut | |
| 700 | 1 | |a Yao, Emmie |e verfasserin |4 aut | |
| 700 | 1 | |a Tang, Min |e verfasserin |4 aut | |
| 700 | 1 | |a Zhong, Zheng |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Xinyue |e verfasserin |4 aut | |
| 700 | 1 | |a Wangpraseurt, Daniel |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Yazhi |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Ting-Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Shaochen |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Science advances |d 2015 |g 9(2023), 8 vom: 22. Feb., Seite eade7923 |w (DE-627)NLM247717614 |x 2375-2548 |7 nnns |
| 773 | 1 | 8 | |g volume:9 |g year:2023 |g number:8 |g day:22 |g month:02 |g pages:eade7923 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1126/sciadv.ade7923 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 9 |j 2023 |e 8 |b 22 |c 02 |h eade7923 | ||