3D Printing as an Effective Quality Assurance Implementation in Massive-Scale SARS-CoV-2 Testing at a SwabSeq Next-Generation Sequencing Laboratory
© American Society for Clinical Pathology 2023. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com..
Massive-scale SARS-CoV-2 testing using the SwabSeq diagnostic platform came with quality assurance challenges due to the novelty and scale of sequencing-based testing. The SwabSeq platform relies on accurate mapping between specimen identifiers and molecular barcodes to match a result back to a patient specimen. To identify and mitigate mapping errors, we instituted quality control using placement of negative controls within a rack of patient samples. We designed 2-dimensional paper templates to fit over a 96-position rack of specimens with holes to show the control tube placements. We designed and 3-dimensionally printed plastic templates that fit onto 4 racks of patient specimens and provide accurate indications of the correct control tube placements. The final plastic templates dramatically reduced plate mapping errors from 22.55% in January 2021 to less than 1% after implementation and training in January 2021. We show how 3D printing can be a cost-effective quality assurance tool to mitigate human error in the clinical laboratory.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:54 |
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| Enthalten in: |
Laboratory medicine - 54(2023), 5 vom: 05. Sept., Seite 512-518 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Sathe, Laila M [VerfasserIn] |
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| Links: |
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| Themen: |
3D printing |
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| Anmerkungen: |
Date Completed 06.09.2023 Date Revised 06.09.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.1093/labmed/lmac161 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a © American Society for Clinical Pathology 2023. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com. | ||
| 520 | |a Massive-scale SARS-CoV-2 testing using the SwabSeq diagnostic platform came with quality assurance challenges due to the novelty and scale of sequencing-based testing. The SwabSeq platform relies on accurate mapping between specimen identifiers and molecular barcodes to match a result back to a patient specimen. To identify and mitigate mapping errors, we instituted quality control using placement of negative controls within a rack of patient samples. We designed 2-dimensional paper templates to fit over a 96-position rack of specimens with holes to show the control tube placements. We designed and 3-dimensionally printed plastic templates that fit onto 4 racks of patient specimens and provide accurate indications of the correct control tube placements. The final plastic templates dramatically reduced plate mapping errors from 22.55% in January 2021 to less than 1% after implementation and training in January 2021. We show how 3D printing can be a cost-effective quality assurance tool to mitigate human error in the clinical laboratory | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a SARS-CoV-2 | |
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| 650 | 4 | |a quality assurance | |
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| 700 | 1 | |a Jajieh, Kane |e verfasserin |4 aut | |
| 700 | 1 | |a Sartippour, Maryam R |e verfasserin |4 aut | |
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| 700 | 1 | |a Arboleda, Valerie A |e verfasserin |4 aut | |
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