Walnut-Derived Peptides Promote Autophagy via the Activation of AMPK/mTOR/ULK1 Pathway to Ameliorate Hyperglycemia in Type 2 Diabetic Mice
Autophagy flux plays a significant protective role in type 2 diabetes mellitus (T2DM). However, the mechanisms by which autophagy mediates insulin resistance (IR) to ameliorate T2DM remain unclear. This study explored the hypoglycemic effects and mechanisms of walnut-derived peptides (fraction 3-10 kDa and LP5) in streptozotocin and high-fat-diet-induced T2DM mice. Findings revealed that walnut-derived peptides reduced the levels of blood glucose and FINS and ameliorated IR and dyslipidemia. They also increased SOD and GSH-PX activities and inhibited the secretion of TNF-α, IL-6, and IL-1β. Additionally, they increased the levels of ATP, COX, SDH, and MMP of liver mitochondria. Western blotting indicated that walnut-derived peptides up-regulated LC3-II/LC3-I and Beclin-1 expression, while they down-regulated p62 expression, which may be associated with the activation of the AMPK/mTOR/ULK1 pathway. Finally, the AMPK activator (AICAR) and inhibitor (Compound C) were used to verify that LP5 could activate autophagy through the AMPK/mTOR/ULK1 pathway in IR HepG2 cells.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:71 |
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| Enthalten in: |
Journal of agricultural and food chemistry - 71(2023), 8 vom: 01. März, Seite 3751-3765 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hou, Weiyu [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 07.03.2023 Date Revised 07.03.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1021/acs.jafc.2c07112 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM353090182 |
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| 520 | |a Autophagy flux plays a significant protective role in type 2 diabetes mellitus (T2DM). However, the mechanisms by which autophagy mediates insulin resistance (IR) to ameliorate T2DM remain unclear. This study explored the hypoglycemic effects and mechanisms of walnut-derived peptides (fraction 3-10 kDa and LP5) in streptozotocin and high-fat-diet-induced T2DM mice. Findings revealed that walnut-derived peptides reduced the levels of blood glucose and FINS and ameliorated IR and dyslipidemia. They also increased SOD and GSH-PX activities and inhibited the secretion of TNF-α, IL-6, and IL-1β. Additionally, they increased the levels of ATP, COX, SDH, and MMP of liver mitochondria. Western blotting indicated that walnut-derived peptides up-regulated LC3-II/LC3-I and Beclin-1 expression, while they down-regulated p62 expression, which may be associated with the activation of the AMPK/mTOR/ULK1 pathway. Finally, the AMPK activator (AICAR) and inhibitor (Compound C) were used to verify that LP5 could activate autophagy through the AMPK/mTOR/ULK1 pathway in IR HepG2 cells | ||
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| 700 | 1 | |a Zhao, Fanrui |e verfasserin |4 aut | |
| 700 | 1 | |a Fang, Li |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiyan |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Dan |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Chunlei |e verfasserin |4 aut | |
| 700 | 1 | |a Leng, Yue |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Yawen |e verfasserin |4 aut | |
| 700 | 1 | |a Fu, Junxi |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Ji |e verfasserin |4 aut | |
| 700 | 1 | |a Min, Weihong |e verfasserin |4 aut | |
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