A novel cryopreservation and biobanking strategy to study lymphoid tissue stromal cells in human disease
Non-hematopoietic lymph node stromal cells (LNSCs) regulate lymphocyte trafficking, survival, and function for key roles in host defense, autoimmunity, alloimmunity, and lymphoproliferative disorders. However, study of LNSCs in human diseases is complicated by a dependence on viable lymphoid tissues, which are most often excised prior to establishment of a specific diagnosis. Here, we demonstrate that cryopreservation can be used to bank lymphoid tissue for the study of LNSCs in human disease. Using human tonsils, lymphoid tissue fragments were cryopreserved for subsequent enzymatic digestion and recovery of viable non-hematopoietic cells. Flow cytometry and single-cell transcriptomics identified comparable proportions of LNSC cell types in fresh and cryopreserved tissue. Moreover, cryopreservation had little effect on transcriptional profiles, which showed significant overlap between tonsils and lymph nodes. The presence and spatial distribution of transcriptionally defined cell types was confirmed by in situ analyses. Our broadly applicable approach promises to greatly enable research into the roles of LNSC in human disease.
| Errataetall: |
UpdateIn: Eur J Immunol. 2023 Jun 27;:e2250362. - PMID 37366295 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2023 |
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| Enthalten in: |
bioRxiv : the preprint server for biology - (2023) vom: 07. Feb. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Brandstadter, Joshua D [VerfasserIn] |
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Date Revised 07.09.2023 published: Electronic UpdateIn: Eur J Immunol. 2023 Jun 27;:e2250362. - PMID 37366295 Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1101/2023.02.06.525604 |
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| PPN (Katalog-ID): |
NLM353048518 |
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| 500 | |a UpdateIn: Eur J Immunol. 2023 Jun 27;:e2250362. - PMID 37366295 | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a Non-hematopoietic lymph node stromal cells (LNSCs) regulate lymphocyte trafficking, survival, and function for key roles in host defense, autoimmunity, alloimmunity, and lymphoproliferative disorders. However, study of LNSCs in human diseases is complicated by a dependence on viable lymphoid tissues, which are most often excised prior to establishment of a specific diagnosis. Here, we demonstrate that cryopreservation can be used to bank lymphoid tissue for the study of LNSCs in human disease. Using human tonsils, lymphoid tissue fragments were cryopreserved for subsequent enzymatic digestion and recovery of viable non-hematopoietic cells. Flow cytometry and single-cell transcriptomics identified comparable proportions of LNSC cell types in fresh and cryopreserved tissue. Moreover, cryopreservation had little effect on transcriptional profiles, which showed significant overlap between tonsils and lymph nodes. The presence and spatial distribution of transcriptionally defined cell types was confirmed by in situ analyses. Our broadly applicable approach promises to greatly enable research into the roles of LNSC in human disease | ||
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| 700 | 1 | |a De Martin, Angelina |e verfasserin |4 aut | |
| 700 | 1 | |a Lütge, Mechthild |e verfasserin |4 aut | |
| 700 | 1 | |a Ferreira, Antonio |e verfasserin |4 aut | |
| 700 | 1 | |a Gaudette, Brian T |e verfasserin |4 aut | |
| 700 | 1 | |a Stanossek, Yves |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Shumei |e verfasserin |4 aut | |
| 700 | 1 | |a Gonzalez, Michael V |e verfasserin |4 aut | |
| 700 | 1 | |a Camiolo, Edward |e verfasserin |4 aut | |
| 700 | 1 | |a Wertheim, Gerald |e verfasserin |4 aut | |
| 700 | 1 | |a Austin, Bridget |e verfasserin |4 aut | |
| 700 | 1 | |a Allman, David |e verfasserin |4 aut | |
| 700 | 1 | |a Lim, Megan S |e verfasserin |4 aut | |
| 700 | 1 | |a Fajgenbaum, David C |e verfasserin |4 aut | |
| 700 | 1 | |a Aster, Jon C |e verfasserin |4 aut | |
| 700 | 1 | |a Ludewig, Burkhard |e verfasserin |4 aut | |
| 700 | 1 | |a Maillard, Ivan |e verfasserin |4 aut | |
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