Modeling angiogenesis in the human brain in a tissue-engineered post-capillary venule
© 2023. The Author(s), under exclusive licence to Springer Nature B.V..
Angiogenesis plays an essential role in embryonic development, organ remodeling, wound healing, and is also associated with many human diseases. The process of angiogenesis in the brain during development is well characterized in animal models, but little is known about the process in the mature brain. Here, we use a tissue-engineered post-capillary venule (PCV) model incorporating stem cell derived induced brain microvascular endothelial-like cells (iBMECs) and pericyte-like cells (iPCs) to visualize the dynamics of angiogenesis. We compare angiogenesis under two conditions: in response to perfusion of growth factors and in the presence of an external concentration gradient. We show that both iBMECs and iPCs can serve as tip cells leading angiogenic sprouts. More importantly, the growth rate for iPC-led sprouts is about twofold higher than for iBMEC-led sprouts. Under a concentration gradient, angiogenic sprouts show a small directional bias toward the high growth factor concentration. Overall, pericytes exhibited a broad range of behavior, including maintaining quiescence, co-migrating with endothelial cells in sprouts, or leading sprout growth as tip cells.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:26 |
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| Enthalten in: |
Angiogenesis - 26(2023), 2 vom: 15. Mai, Seite 203-216 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhao, Nan [VerfasserIn] |
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| Links: |
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| Themen: |
Angiogenesis |
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| Anmerkungen: |
Date Completed 24.04.2023 Date Revised 10.02.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s10456-023-09868-7 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM353018104 |
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| 520 | |a Angiogenesis plays an essential role in embryonic development, organ remodeling, wound healing, and is also associated with many human diseases. The process of angiogenesis in the brain during development is well characterized in animal models, but little is known about the process in the mature brain. Here, we use a tissue-engineered post-capillary venule (PCV) model incorporating stem cell derived induced brain microvascular endothelial-like cells (iBMECs) and pericyte-like cells (iPCs) to visualize the dynamics of angiogenesis. We compare angiogenesis under two conditions: in response to perfusion of growth factors and in the presence of an external concentration gradient. We show that both iBMECs and iPCs can serve as tip cells leading angiogenic sprouts. More importantly, the growth rate for iPC-led sprouts is about twofold higher than for iBMEC-led sprouts. Under a concentration gradient, angiogenic sprouts show a small directional bias toward the high growth factor concentration. Overall, pericytes exhibited a broad range of behavior, including maintaining quiescence, co-migrating with endothelial cells in sprouts, or leading sprout growth as tip cells | ||
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| 650 | 4 | |a Angiogenesis | |
| 650 | 4 | |a Angiogenic sprout | |
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| 650 | 4 | |a Pericytes | |
| 650 | 4 | |a Tip cell | |
| 650 | 4 | |a Tissue engineering | |
| 700 | 1 | |a Kulkarni, Sarah |e verfasserin |4 aut | |
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| 700 | 1 | |a Linville, Raleigh M |e verfasserin |4 aut | |
| 700 | 1 | |a Chung, Tracy D |e verfasserin |4 aut | |
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| 700 | 1 | |a Pessell, Alexander F |e verfasserin |4 aut | |
| 700 | 1 | |a Searson, Peter |e verfasserin |4 aut | |
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