Establishment of a Pseudovirus Platform for Neuraminidase Inhibiting Antibody Analysis
Neuraminidase (NA)-based immunity to influenza can be useful for protecting against novel antigenic variants. To develop safe and effective tools to assess NA-based immunity, we generated a baculovirus-based pseudotyped virus, N1-Bac, that expresses the full-length NA of the influenza A/California/07/2009 (H1N1)pdm09 strain. We evaluated the level of NA-inhibiting (NI) antibodies in the paired blood sera of influenza patients by means of an enzyme-linked lectin assay (ELLA) using the influenza virus or N1-Bac. Additionally, we evaluated the level of NA antibodies by means of the enzyme-linked immunosorbent assay (ELISA) with an N1-expressing Sf21 culture. We detected a strong correlation between our results from using the influenza virus and NA-Bac pseudoviruses to detect NI antibodies and a medium-strong correlation between NI antibodies and NA antibodies determined by an N1-cell ELISA, indicating that baculovirus-based platforms can be successfully used to evaluate NI or NA antibodies. Furthermore, animal experiments showed that immunization with N1-Bac protected against infection with a drift variant of the A/H1N1pdm09 influenza virus. Our results demonstrate that recombinant baculovirus can be an effective influenza pseudotype to evaluate influenza serologic immunity and protect against influenza virus infection.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:24 |
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| Enthalten in: |
International journal of molecular sciences - 24(2023), 3 vom: 25. Jan. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Desheva, Yulia [VerfasserIn] |
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| Links: |
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| Themen: |
Antibodies, Blocking |
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| Anmerkungen: |
Date Completed 14.02.2023 Date Revised 14.02.2023 published: Electronic Citation Status MEDLINE |
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| doi: |
10.3390/ijms24032376 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM352784121 |
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| 245 | 1 | 0 | |a Establishment of a Pseudovirus Platform for Neuraminidase Inhibiting Antibody Analysis |
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| 520 | |a Neuraminidase (NA)-based immunity to influenza can be useful for protecting against novel antigenic variants. To develop safe and effective tools to assess NA-based immunity, we generated a baculovirus-based pseudotyped virus, N1-Bac, that expresses the full-length NA of the influenza A/California/07/2009 (H1N1)pdm09 strain. We evaluated the level of NA-inhibiting (NI) antibodies in the paired blood sera of influenza patients by means of an enzyme-linked lectin assay (ELLA) using the influenza virus or N1-Bac. Additionally, we evaluated the level of NA antibodies by means of the enzyme-linked immunosorbent assay (ELISA) with an N1-expressing Sf21 culture. We detected a strong correlation between our results from using the influenza virus and NA-Bac pseudoviruses to detect NI antibodies and a medium-strong correlation between NI antibodies and NA antibodies determined by an N1-cell ELISA, indicating that baculovirus-based platforms can be successfully used to evaluate NI or NA antibodies. Furthermore, animal experiments showed that immunization with N1-Bac protected against infection with a drift variant of the A/H1N1pdm09 influenza virus. Our results demonstrate that recombinant baculovirus can be an effective influenza pseudotype to evaluate influenza serologic immunity and protect against influenza virus infection | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Sf21 cell culture | |
| 650 | 4 | |a baculovirus | |
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| 650 | 4 | |a neuraminidase-inhibiting antibodies | |
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| 650 | 7 | |a Neuraminidase |2 NLM | |
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| 650 | 7 | |a Antibodies, Viral |2 NLM | |
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| 650 | 7 | |a Influenza Vaccines |2 NLM | |
| 700 | 1 | |a Petkova, Nadezhda |e verfasserin |4 aut | |
| 700 | 1 | |a Losev, Igor |e verfasserin |4 aut | |
| 700 | 1 | |a Guzhov, Dmitry |e verfasserin |4 aut | |
| 700 | 1 | |a Go, Alexey |e verfasserin |4 aut | |
| 700 | 1 | |a Chao, Yu-Chan |e verfasserin |4 aut | |
| 700 | 1 | |a Tsai, Chih-Hsuan |e verfasserin |4 aut | |
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