Caco-2 cell-derived biomimetic electrochemical biosensor for cholera toxin detection
Copyright © 2023 Elsevier B.V. All rights reserved..
Cholera is a highly contagious and lethal waterborne disease induced by an infection with Vibrio cholerae (V. cholerae) secreting cholera toxin (CTx). Cholera toxin subunit B (CTxB) from the CTx specifically binds with monosialo-tetra-hexosyl-ganglioside (GM1) found on the exterior cell membrane of an enterocyte. Bioinspired by the pathological process of CTx, we developed an electrochemical biosensor with GM1-expressing Caco-2 cell membrane (CCM) on the electrode surface. Briefly, the electrode surface was functionalized with CCM using the vesicle fusion method. We determined the CTxB detection performances of Caco-2 cell membrane-coated biosensor (CCB) using electrochemical impedance spectroscopy (EIS). the CCB had an excellent limit of detection of ∼11.46 nM and a detection range spanning 100 ng/mL - 1 mg/mL. In addition, the CCB showed high selectivity against various interfering molecules, including abundant constituents of intestinal fluid and various bacterial toxins. The long-term stability of the CCBs was also verified for 3 weeks using EIS. Overall, the CCB has excellent potential for practical use such as point-of-care and cost-effective testing for CTxB detection in developing countries.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:226 |
|---|---|
| Enthalten in: |
Biosensors & bioelectronics - 226(2023) vom: 15. Apr., Seite 115105 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Kim, Yonghwan [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
37758-47-7 |
|---|
| Anmerkungen: |
Date Completed 24.02.2023 Date Revised 24.02.2023 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.bios.2023.115105 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM352559950 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM352559950 | ||
| 003 | DE-627 | ||
| 005 | 20231226054015.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.bios.2023.115105 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1175.xml |
| 035 | |a (DE-627)NLM352559950 | ||
| 035 | |a (NLM)36746024 | ||
| 035 | |a (PII)S0956-5663(23)00047-7 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Kim, Yonghwan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Caco-2 cell-derived biomimetic electrochemical biosensor for cholera toxin detection |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 24.02.2023 | ||
| 500 | |a Date Revised 24.02.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 Elsevier B.V. All rights reserved. | ||
| 520 | |a Cholera is a highly contagious and lethal waterborne disease induced by an infection with Vibrio cholerae (V. cholerae) secreting cholera toxin (CTx). Cholera toxin subunit B (CTxB) from the CTx specifically binds with monosialo-tetra-hexosyl-ganglioside (GM1) found on the exterior cell membrane of an enterocyte. Bioinspired by the pathological process of CTx, we developed an electrochemical biosensor with GM1-expressing Caco-2 cell membrane (CCM) on the electrode surface. Briefly, the electrode surface was functionalized with CCM using the vesicle fusion method. We determined the CTxB detection performances of Caco-2 cell membrane-coated biosensor (CCB) using electrochemical impedance spectroscopy (EIS). the CCB had an excellent limit of detection of ∼11.46 nM and a detection range spanning 100 ng/mL - 1 mg/mL. In addition, the CCB showed high selectivity against various interfering molecules, including abundant constituents of intestinal fluid and various bacterial toxins. The long-term stability of the CCBs was also verified for 3 weeks using EIS. Overall, the CCB has excellent potential for practical use such as point-of-care and cost-effective testing for CTxB detection in developing countries | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Bioinspired | |
| 650 | 4 | |a Biosensor | |
| 650 | 4 | |a Caco-2 cell membrane | |
| 650 | 4 | |a Cholera | |
| 650 | 4 | |a Cholera toxin subunit B | |
| 650 | 4 | |a Electrochemical impedance spectroscopy | |
| 650 | 7 | |a Cholera Toxin |2 NLM | |
| 650 | 7 | |a 9012-63-9 |2 NLM | |
| 650 | 7 | |a G(M1) Ganglioside |2 NLM | |
| 650 | 7 | |a 37758-47-7 |2 NLM | |
| 700 | 1 | |a Lee, Dongtak |e verfasserin |4 aut | |
| 700 | 1 | |a Seo, Youngjun |e verfasserin |4 aut | |
| 700 | 1 | |a Jung, Hyo Gi |e verfasserin |4 aut | |
| 700 | 1 | |a Jang, Jae Won |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Dongsung |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Insu |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Jaeheung |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Gyudo |e verfasserin |4 aut | |
| 700 | 1 | |a Hwang, Kyo Seon |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Seung-Hyun |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Sang Won |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Jeong Hoon |e verfasserin |4 aut | |
| 700 | 1 | |a Yoon, Dae Sung |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biosensors & bioelectronics |d 1996 |g 226(2023) vom: 15. Apr., Seite 115105 |w (DE-627)NLM012733431 |x 1873-4235 |7 nnns |
| 773 | 1 | 8 | |g volume:226 |g year:2023 |g day:15 |g month:04 |g pages:115105 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.bios.2023.115105 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 226 |j 2023 |b 15 |c 04 |h 115105 | ||