Detection of IgG protein in human urine based on vertical flow paper microfluidic chip
The kidney is the body's most important organ and the protein components in urine could be detected for diagnosing certain diseases. The amount of IgG protein in urine could be used to determine the degree of kidney function damage. IgG protein in human urine was detected by vertical flow paper-based microfluidic chip, double-antibody sandwich immunoreaction, and cell phone image processing. The results showed that using an IgG antibody concentration of 500 μg/mL and a gold standard antibody concentration of 100 μg/mL, the image signal showed a good linear relationship in the range of IgG concentration of 0.2-3.2 μg/mL, with R2=0.973 3 achieved. A complete set of detection devices were designed and the detection method showed good non-specificity.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:39 |
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| Enthalten in: |
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 39(2023), 1 vom: 25. Jan., Seite 337-346 |
| Sprache: |
Chinesisch |
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| Beteiligte Personen: |
Li, Xinru [VerfasserIn] |
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| Links: |
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| Themen: |
Detection device |
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| Anmerkungen: |
Date Completed 07.02.2023 Date Revised 07.02.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.13345/j.cjb.220246 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM352482400 |
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| 245 | 1 | 0 | |a Detection of IgG protein in human urine based on vertical flow paper microfluidic chip |
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| 500 | |a Date Revised 07.02.2023 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The kidney is the body's most important organ and the protein components in urine could be detected for diagnosing certain diseases. The amount of IgG protein in urine could be used to determine the degree of kidney function damage. IgG protein in human urine was detected by vertical flow paper-based microfluidic chip, double-antibody sandwich immunoreaction, and cell phone image processing. The results showed that using an IgG antibody concentration of 500 μg/mL and a gold standard antibody concentration of 100 μg/mL, the image signal showed a good linear relationship in the range of IgG concentration of 0.2-3.2 μg/mL, with R2=0.973 3 achieved. A complete set of detection devices were designed and the detection method showed good non-specificity | ||
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a detection device | |
| 650 | 4 | |a detection of IgG protein | |
| 650 | 4 | |a human urine | |
| 650 | 4 | |a image processing | |
| 650 | 4 | |a paper-based microfluidic devices | |
| 650 | 4 | |a vertical flow | |
| 650 | 7 | |a Immunoglobulin G |2 NLM | |
| 700 | 1 | |a Wang, Xinyi |e verfasserin |4 aut | |
| 700 | 1 | |a Wei, Ziyu |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Penghui |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Jingwen |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Lang |e verfasserin |4 aut | |
| 700 | 1 | |a Zheng, Feifan |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Zhenwei |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Yuanyuan |e verfasserin |4 aut | |
| 700 | 1 | |a Qiu, Xianbo |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Lulu |e verfasserin |4 aut | |
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