Structural and functional integration of human forebrain organoids with the injured adult rat visual system
Published by Elsevier Inc..
Brain organoids created from human pluripotent stem cells represent a promising approach for brain repair. They acquire many structural features of the brain and raise the possibility of patient-matched repair. Whether these entities can integrate with host brain networks in the context of the injured adult mammalian brain is not well established. Here, we provide structural and functional evidence that human brain organoids successfully integrate with the adult rat visual system after transplantation into large injury cavities in the visual cortex. Virus-based trans-synaptic tracing reveals a polysynaptic pathway between organoid neurons and the host retina and reciprocal connectivity between the graft and other regions of the visual system. Visual stimulation of host animals elicits responses in organoid neurons, including orientation selectivity. These results demonstrate the ability of human brain organoids to adopt sophisticated function after insertion into large injury cavities, suggesting a translational strategy to restore function after cortical damage.
| Errataetall: |
CommentIn: Cell Stem Cell. 2023 Mar 2;30(3):241-242. - PMID 36764295 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:30 |
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| Enthalten in: |
Cell stem cell - 30(2023), 2 vom: 02. Feb., Seite 137-152.e7 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jgamadze, Dennis [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 07.02.2023 Date Revised 03.02.2024 published: Print CommentIn: Cell Stem Cell. 2023 Mar 2;30(3):241-242. - PMID 36764295 Citation Status MEDLINE |
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| doi: |
10.1016/j.stem.2023.01.004 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 500 | |a Date Revised 03.02.2024 | ||
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| 500 | |a CommentIn: Cell Stem Cell. 2023 Mar 2;30(3):241-242. - PMID 36764295 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Published by Elsevier Inc. | ||
| 520 | |a Brain organoids created from human pluripotent stem cells represent a promising approach for brain repair. They acquire many structural features of the brain and raise the possibility of patient-matched repair. Whether these entities can integrate with host brain networks in the context of the injured adult mammalian brain is not well established. Here, we provide structural and functional evidence that human brain organoids successfully integrate with the adult rat visual system after transplantation into large injury cavities in the visual cortex. Virus-based trans-synaptic tracing reveals a polysynaptic pathway between organoid neurons and the host retina and reciprocal connectivity between the graft and other regions of the visual system. Visual stimulation of host animals elicits responses in organoid neurons, including orientation selectivity. These results demonstrate the ability of human brain organoids to adopt sophisticated function after insertion into large injury cavities, suggesting a translational strategy to restore function after cortical damage | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 4 | |a brain organoid | |
| 650 | 4 | |a brain repair | |
| 650 | 4 | |a functional integration | |
| 650 | 4 | |a stem cells | |
| 650 | 4 | |a visual cortex | |
| 700 | 1 | |a Lim, James T |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Zhijian |e verfasserin |4 aut | |
| 700 | 1 | |a Harary, Paul M |e verfasserin |4 aut | |
| 700 | 1 | |a Germi, James |e verfasserin |4 aut | |
| 700 | 1 | |a Mensah-Brown, Kobina |e verfasserin |4 aut | |
| 700 | 1 | |a Adam, Christopher D |e verfasserin |4 aut | |
| 700 | 1 | |a Mirzakhalili, Ehsan |e verfasserin |4 aut | |
| 700 | 1 | |a Singh, Shikha |e verfasserin |4 aut | |
| 700 | 1 | |a Gu, Jiahe Ben |e verfasserin |4 aut | |
| 700 | 1 | |a Blue, Rachel |e verfasserin |4 aut | |
| 700 | 1 | |a Dedhia, Mehek |e verfasserin |4 aut | |
| 700 | 1 | |a Fu, Marissa |e verfasserin |4 aut | |
| 700 | 1 | |a Jacob, Fadi |e verfasserin |4 aut | |
| 700 | 1 | |a Qian, Xuyu |e verfasserin |4 aut | |
| 700 | 1 | |a Gagnon, Kimberly |e verfasserin |4 aut | |
| 700 | 1 | |a Sergison, Matthew |e verfasserin |4 aut | |
| 700 | 1 | |a Fruchet, Oceane |e verfasserin |4 aut | |
| 700 | 1 | |a Rahaman, Imon |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Huadong |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Fuqiang |e verfasserin |4 aut | |
| 700 | 1 | |a Xiao, Rui |e verfasserin |4 aut | |
| 700 | 1 | |a Contreras, Diego |e verfasserin |4 aut | |
| 700 | 1 | |a Wolf, John A |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Hongjun |e verfasserin |4 aut | |
| 700 | 1 | |a Ming, Guo-Li |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Han-Chiao Isaac |e verfasserin |4 aut | |
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