PolyIC-coated Prussian blue nanoparticles as a dual-mode HIV latency reversing agent
Aim: To investigate Prussian blue nanoparticles (PBNPs) coated with the synthetic analog of dsRNA polyinosinic-polycytidylic acid (polyIC) for their ability to function as HIV latency reversing agents. Methods: A layer-by-layer method was used to synthesize polyIC-coated PBNPs (polyIC-PBNPs). PolyIC-PBNPs were stable and monodisperse, maintained the native absorbance properties of both polyIC and PBNPs and were obtained with high nanoparticle collection yield and polyIC attachment efficiencies. Results: PolyIC-PBNPs were more effective in reactivating latent HIV than free polyIC in a cell model of HIV latency. Furthermore, polyIC-PBNPs were more effective in promoting immune activation than free polyIC in CD4 and CD8 T cells. Conclusion: PBNPs function as efficient carriers of nucleic acids to directly reverse HIV latency and enhance immune activation.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:17 |
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| Enthalten in: |
Nanomedicine (London, England) - 17(2022), 29 vom: 22. Dez., Seite 2159-2171 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Balakrishnan, Preethi B [VerfasserIn] |
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| Links: |
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| Themen: |
Ferric ferrocyanide |
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| Anmerkungen: |
Date Completed 30.03.2023 Date Revised 02.12.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.2217/nnm-2022-0311 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM352445289 |
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| 245 | 1 | 0 | |a PolyIC-coated Prussian blue nanoparticles as a dual-mode HIV latency reversing agent |
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| 500 | |a Date Revised 02.12.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Aim: To investigate Prussian blue nanoparticles (PBNPs) coated with the synthetic analog of dsRNA polyinosinic-polycytidylic acid (polyIC) for their ability to function as HIV latency reversing agents. Methods: A layer-by-layer method was used to synthesize polyIC-coated PBNPs (polyIC-PBNPs). PolyIC-PBNPs were stable and monodisperse, maintained the native absorbance properties of both polyIC and PBNPs and were obtained with high nanoparticle collection yield and polyIC attachment efficiencies. Results: PolyIC-PBNPs were more effective in reactivating latent HIV than free polyIC in a cell model of HIV latency. Furthermore, polyIC-PBNPs were more effective in promoting immune activation than free polyIC in CD4 and CD8 T cells. Conclusion: PBNPs function as efficient carriers of nucleic acids to directly reverse HIV latency and enhance immune activation | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a HIV • HIV latent reservoirs | |
| 650 | 4 | |a Prussian blue nanoparticles | |
| 650 | 4 | |a latency reversing agents | |
| 650 | 4 | |a polyinosinic-polycytidylic acid | |
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| 700 | 1 | |a Ledezma, Debbie K |e verfasserin |4 aut | |
| 700 | 1 | |a Bosque, Alberto |e verfasserin |4 aut | |
| 700 | 1 | |a Fernandes, Rohan |e verfasserin |4 aut | |
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