Biological and metabolic effects of the association between the microalga Galdieria sulphuraria and the fungus Penicillium citrinum
© 2023. The Author(s)..
Contamination of microalgae cultures can reduce their productivity and affect the quality of biomass and valuable bioproducts. In this article, after having isolated and identified for the first time the filamentous fungus Penicillium citrinum from heterotrophic cultures of the red polyextremophilic microalga Galdieria sulphuraria, we investigated the biological and metabolic significance of this alga-fungus association. In the same medium, both organisms grow better in each other's presence than separately. Both cell density and cell size of G. sulphuraria increase in co-cultures compared to pure alga cultures. In co-cultures, despite very severe growth conditions, the load of P. citrinum increases compared to pure fungus cultures. Optical microscope images have shown physical contact between cells of P. citrinum hyphae and G. sulphuraria which, however, retain their morphology and cell wall intact. GC-MS-based metabolomics analysis of metabolites excreted in the culture medium shows that pure cultures of the fungus and alga and co-cultures of alga plus fungus can be easily differentiated based on their metabolic products. Indeed, a richer assortment of extracellular metabolites (comprising both products of primary and secondary metabolism) is a distinct feature of co-cultures compared to both pure alga and pure fungus cultures.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Scientific reports - 13(2023), 1 vom: 31. Jan., Seite 1789 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Salvatore, Maria Michela [VerfasserIn] |
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| Anmerkungen: |
Date Completed 02.02.2023 Date Revised 16.03.2023 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41598-023-27827-6 |
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| Förderinstitution / Projekttitel: |
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NLM352313153 |
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| 245 | 1 | 0 | |a Biological and metabolic effects of the association between the microalga Galdieria sulphuraria and the fungus Penicillium citrinum |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023. The Author(s). | ||
| 520 | |a Contamination of microalgae cultures can reduce their productivity and affect the quality of biomass and valuable bioproducts. In this article, after having isolated and identified for the first time the filamentous fungus Penicillium citrinum from heterotrophic cultures of the red polyextremophilic microalga Galdieria sulphuraria, we investigated the biological and metabolic significance of this alga-fungus association. In the same medium, both organisms grow better in each other's presence than separately. Both cell density and cell size of G. sulphuraria increase in co-cultures compared to pure alga cultures. In co-cultures, despite very severe growth conditions, the load of P. citrinum increases compared to pure fungus cultures. Optical microscope images have shown physical contact between cells of P. citrinum hyphae and G. sulphuraria which, however, retain their morphology and cell wall intact. GC-MS-based metabolomics analysis of metabolites excreted in the culture medium shows that pure cultures of the fungus and alga and co-cultures of alga plus fungus can be easily differentiated based on their metabolic products. Indeed, a richer assortment of extracellular metabolites (comprising both products of primary and secondary metabolism) is a distinct feature of co-cultures compared to both pure alga and pure fungus cultures | ||
| 650 | 4 | |a Journal Article | |
| 700 | 1 | |a Carraturo, Federica |e verfasserin |4 aut | |
| 700 | 1 | |a Salbitani, Giovanna |e verfasserin |4 aut | |
| 700 | 1 | |a Rosati, Luigi |e verfasserin |4 aut | |
| 700 | 1 | |a De Risi, Arianna |e verfasserin |4 aut | |
| 700 | 1 | |a Andolfi, Anna |e verfasserin |4 aut | |
| 700 | 1 | |a Salvatore, Francesco |e verfasserin |4 aut | |
| 700 | 1 | |a Guida, Marco |e verfasserin |4 aut | |
| 700 | 1 | |a Carfagna, Simona |e verfasserin |4 aut | |
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