T-independent responses to polysaccharides in humans mobilize marginal zone B cells prediversified against gut bacterial antigens
Marginal zone (MZ) B cells are one of the main actors of T-independent (TI) responses in mice. To identify the B cell subset(s) involved in such responses in humans, we vaccinated healthy individuals with Pneumovax, a model TI vaccine. By high-throughput repertoire sequencing of plasma cells (PCs) isolated 7 days after vaccination and of different B cell subpopulations before and after vaccination, we show that the PC response mobilizes large clones systematically, including an immunoglobulin M component, whose diversification and amplification predated the pneumococcal vaccination. These clones could be mainly traced back to MZ B cells, together with clonally related IgA+ and, to a lesser extent, IgG+CD27+ B cells. Recombinant monoclonal antibodies isolated from large PC clones recognized a wide array of bacterial species from the gut flora, indicating that TI responses in humans largely mobilize MZ and switched B cells that most likely prediversified during mucosal immune responses against bacterial antigens and acquired pneumococcal cross-reactivity through somatic hypermutation.
| Errataetall: |
CommentIn: Immunol Lett. 2023 Apr-May;256-257:66-67. - PMID 37040847 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:8 |
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| Enthalten in: |
Science immunology - 8(2023), 79 vom: 27. Jan., Seite eade1413 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Weller, Sandra [VerfasserIn] |
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| Links: |
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| Themen: |
Antigens, Bacterial |
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| Anmerkungen: |
Date Completed 03.02.2023 Date Revised 03.10.2023 published: Print-Electronic CommentIn: Immunol Lett. 2023 Apr-May;256-257:66-67. - PMID 37040847 Citation Status MEDLINE |
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| doi: |
10.1126/sciimmunol.ade1413 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 500 | |a CommentIn: Immunol Lett. 2023 Apr-May;256-257:66-67. - PMID 37040847 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Marginal zone (MZ) B cells are one of the main actors of T-independent (TI) responses in mice. To identify the B cell subset(s) involved in such responses in humans, we vaccinated healthy individuals with Pneumovax, a model TI vaccine. By high-throughput repertoire sequencing of plasma cells (PCs) isolated 7 days after vaccination and of different B cell subpopulations before and after vaccination, we show that the PC response mobilizes large clones systematically, including an immunoglobulin M component, whose diversification and amplification predated the pneumococcal vaccination. These clones could be mainly traced back to MZ B cells, together with clonally related IgA+ and, to a lesser extent, IgG+CD27+ B cells. Recombinant monoclonal antibodies isolated from large PC clones recognized a wide array of bacterial species from the gut flora, indicating that TI responses in humans largely mobilize MZ and switched B cells that most likely prediversified during mucosal immune responses against bacterial antigens and acquired pneumococcal cross-reactivity through somatic hypermutation | ||
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| 650 | 7 | |a Antigens, Bacterial |2 NLM | |
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| 650 | 7 | |a Polysaccharides |2 NLM | |
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| 700 | 1 | |a Verge de Los Aires, Alba |e verfasserin |4 aut | |
| 700 | 1 | |a Goetz, Clara |e verfasserin |4 aut | |
| 700 | 1 | |a Fritzen, Rémi |e verfasserin |4 aut | |
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| 700 | 1 | |a Weill, Jean-Claude |e verfasserin |4 aut | |
| 700 | 1 | |a Reynaud, Claude-Agnès |e verfasserin |4 aut | |
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