MicroRNA Identification, Target Prediction, and Validation for Crop Improvement
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature..
Micro-RNAs (mi-RNAs) are regulatory elements that play a vital role in the growth, development, and metabolic regulation of plants. In current research, the isolation of miRNAs is a tedious and difficult task using in vitro methods. However, recent exploration into the remarkably highly conserved nature of nucleotide sequences of miRNAs assists in the identification of miRNAs in plant species through homologous approaches. Here, we describe the in silico-based method for identification of miRNAs from the EST database which is emerging as a faster and more reliable approach along with the development of miRNA-SSR markers. This approach has the potential to accelerate research into the regulation of gene expression in various plant species such as tea, potato, tomato, tobacco, and orphan crops like cluster bean.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2630 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2630(2023) vom: 03., Seite 13-24 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chaudhary, Vrantika [VerfasserIn] |
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| Links: |
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| Themen: |
Diversity analysis |
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| Anmerkungen: |
Date Completed 25.01.2023 Date Revised 13.12.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-2982-6_2 |
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| funding: |
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| 520 | |a Micro-RNAs (mi-RNAs) are regulatory elements that play a vital role in the growth, development, and metabolic regulation of plants. In current research, the isolation of miRNAs is a tedious and difficult task using in vitro methods. However, recent exploration into the remarkably highly conserved nature of nucleotide sequences of miRNAs assists in the identification of miRNAs in plant species through homologous approaches. Here, we describe the in silico-based method for identification of miRNAs from the EST database which is emerging as a faster and more reliable approach along with the development of miRNA-SSR markers. This approach has the potential to accelerate research into the regulation of gene expression in various plant species such as tea, potato, tomato, tobacco, and orphan crops like cluster bean | ||
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