Escaping Behavior of Sperms on the Biomimetic Oviductal Surface
Before fertilization, sperms adhere to oviductal epithelium cells, and only a restrictive number of winner sperms can escape to reach the egg. To study the sperm escape behavior from the oviductal surface, we developed a microfluidic chip to fabricate an adhesive surface and to create a gradient of progesterone (P4) for mimicking the oviduct microenvironment in vivo. We identified three sperm motion patterns in such a microenvironment─anchored spin, run-and-spin, and escaped mode. By using kinetic analysis, we verified the hypothesis that the responsive rotation energy anchored with the adhered sperm head determines whether the sperm is trapped or detaching, which is defined as the hammer flying strategy of successful escape after accumulating energy in the process of rotating. Intriguingly, this hammer-throw escaping is able to be triggered by the P4 biochemical stimulation. Our results revealed the tangled process of sperm escape before fertilization in the ingenious microfluidic system.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:95 |
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| Enthalten in: |
Analytical chemistry - 95(2023), 4 vom: 31. Jan., Seite 2366-2374 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yu, Sai-Xi [VerfasserIn] |
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| Anmerkungen: |
Date Completed 01.02.2023 Date Revised 21.02.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1021/acs.analchem.2c04338 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM351663665 |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Before fertilization, sperms adhere to oviductal epithelium cells, and only a restrictive number of winner sperms can escape to reach the egg. To study the sperm escape behavior from the oviductal surface, we developed a microfluidic chip to fabricate an adhesive surface and to create a gradient of progesterone (P4) for mimicking the oviduct microenvironment in vivo. We identified three sperm motion patterns in such a microenvironment─anchored spin, run-and-spin, and escaped mode. By using kinetic analysis, we verified the hypothesis that the responsive rotation energy anchored with the adhered sperm head determines whether the sperm is trapped or detaching, which is defined as the hammer flying strategy of successful escape after accumulating energy in the process of rotating. Intriguingly, this hammer-throw escaping is able to be triggered by the P4 biochemical stimulation. Our results revealed the tangled process of sperm escape before fertilization in the ingenious microfluidic system | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 700 | 1 | |a Wu, Yi |e verfasserin |4 aut | |
| 700 | 1 | |a Luo, Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yanan |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Yu-Chen |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Ya-Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Tang, Jianan |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Huijuan |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Hai |e verfasserin |4 aut | |
| 700 | 1 | |a Jing, Guangyin |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yan-Jun |e verfasserin |4 aut | |
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