Mutation of DEFECTIVE EMBRYO SAC1 results in a low seed-setting rate in rice by regulating embryo sac development
© The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology..
The seed-setting rate has a significant effect on grain yield in rice (Oryza sativa L.). Embryo sac development is essential for seed setting; however, the molecular mechanism underlying this process remains unclear. Here, we isolated defective embryo sac1 (des1), a rice mutant with a low seed-setting rate. Cytological examination showed degenerated embryo sacs and reduced fertilization capacity in des1. Map-based cloning revealed a nonsense mutation in OsDES1, a gene that encodes a putative nuclear envelope membrane protein (NEMP)-domain-containing protein that is preferentially expressed in pistils. The OsDES1 mutation disrupts the normal formation of functional megaspores, which ultimately results in a degenerated embryo sac in des1. Reciprocal crosses showed that fertilization is abnormal and that the female reproductive organ is defective in des1. OsDES1 interacts with LONELY GUY (LOG), a cytokinin-activating enzyme that acts in the final step of cytokinin synthesis; mutation of LOG led to defective female reproductive organ development. These results demonstrate that OsDES1 functions in determining the rice seed-setting rate by regulating embryo sac development and fertilization. Our study sheds light on the function of NEMP-type proteins in rice reproductive development.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:74 |
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| Enthalten in: |
Journal of experimental botany - 74(2023), 5 vom: 13. März, Seite 1501-1516 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hu, Xia [VerfasserIn] |
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| Links: |
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| Themen: |
Embryo sac |
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| Anmerkungen: |
Date Completed 15.03.2023 Date Revised 04.04.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.1093/jxb/erac506 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 0 | |a Mutation of DEFECTIVE EMBRYO SAC1 results in a low seed-setting rate in rice by regulating embryo sac development |
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| 520 | |a © The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology. | ||
| 520 | |a The seed-setting rate has a significant effect on grain yield in rice (Oryza sativa L.). Embryo sac development is essential for seed setting; however, the molecular mechanism underlying this process remains unclear. Here, we isolated defective embryo sac1 (des1), a rice mutant with a low seed-setting rate. Cytological examination showed degenerated embryo sacs and reduced fertilization capacity in des1. Map-based cloning revealed a nonsense mutation in OsDES1, a gene that encodes a putative nuclear envelope membrane protein (NEMP)-domain-containing protein that is preferentially expressed in pistils. The OsDES1 mutation disrupts the normal formation of functional megaspores, which ultimately results in a degenerated embryo sac in des1. Reciprocal crosses showed that fertilization is abnormal and that the female reproductive organ is defective in des1. OsDES1 interacts with LONELY GUY (LOG), a cytokinin-activating enzyme that acts in the final step of cytokinin synthesis; mutation of LOG led to defective female reproductive organ development. These results demonstrate that OsDES1 functions in determining the rice seed-setting rate by regulating embryo sac development and fertilization. Our study sheds light on the function of NEMP-type proteins in rice reproductive development | ||
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| 700 | 1 | |a Yu, Ping |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yingxin |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Zhiqiang |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Bin |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Weixun |e verfasserin |4 aut | |
| 700 | 1 | |a Deng, Chenwei |e verfasserin |4 aut | |
| 700 | 1 | |a Abbas, Adil |e verfasserin |4 aut | |
| 700 | 1 | |a Hong, Yongbo |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Lianping |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Qunen |e verfasserin |4 aut | |
| 700 | 1 | |a Xue, Pao |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Beifang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhan, Xiaodeng |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Liyong |e verfasserin |4 aut | |
| 700 | 1 | |a Cheng, Shihua |e verfasserin |4 aut | |
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