Spike-specific T cells are enriched in breastmilk following SARS-CoV-2 mRNA vaccination
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved..
Human breastmilk is rich in T cells; however, their specificity and function are largely unknown. We compared the phenotype, diversity, and antigen specificity of T cells in breastmilk and peripheral blood of lactating individuals who received SARS-CoV-2 messenger RNA (mRNA) vaccination. Relative to blood, breastmilk contained higher frequencies of T effector and central memory populations that expressed mucosal-homing markers. T cell receptor sequence overlap was limited between blood and breastmilk. Overabundant breastmilk clones were observed in all individuals, were diverse, and contained complementarity-determining regions in three sequences with known epitope specificity, including to SARS-CoV-2 spike. SARS-CoV-2 spike-specific T cell receptors were more frequent in breastmilk compared to blood and expanded in breastmilk following a 3rd mRNA vaccine dose. Our observations indicate that the lactating breast contains a distinct T cell population that can be modulated by maternal vaccination with potential implications for passive infant protection.
| Errataetall: | |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:16 |
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| Enthalten in: |
Mucosal immunology - 16(2023), 1 vom: 21. Feb., Seite 39-49 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Armistead, Blair [VerfasserIn] |
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| Links: |
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| Themen: |
Antibodies, Viral |
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| Anmerkungen: |
Date Completed 14.03.2023 Date Revised 01.03.2024 published: Print-Electronic UpdateOf: medRxiv. 2022 Sep 28;:. - PMID 36203549 Citation Status MEDLINE |
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| doi: |
10.1016/j.mucimm.2023.01.003 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a Human breastmilk is rich in T cells; however, their specificity and function are largely unknown. We compared the phenotype, diversity, and antigen specificity of T cells in breastmilk and peripheral blood of lactating individuals who received SARS-CoV-2 messenger RNA (mRNA) vaccination. Relative to blood, breastmilk contained higher frequencies of T effector and central memory populations that expressed mucosal-homing markers. T cell receptor sequence overlap was limited between blood and breastmilk. Overabundant breastmilk clones were observed in all individuals, were diverse, and contained complementarity-determining regions in three sequences with known epitope specificity, including to SARS-CoV-2 spike. SARS-CoV-2 spike-specific T cell receptors were more frequent in breastmilk compared to blood and expanded in breastmilk following a 3rd mRNA vaccine dose. Our observations indicate that the lactating breast contains a distinct T cell population that can be modulated by maternal vaccination with potential implications for passive infant protection | ||
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| 700 | 1 | |a Jani, Saumya |e verfasserin |4 aut | |
| 700 | 1 | |a Houck, John |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Xia |e verfasserin |4 aut | |
| 700 | 1 | |a Jing, Lichen |e verfasserin |4 aut | |
| 700 | 1 | |a Pecor, Tiffany |e verfasserin |4 aut | |
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| 700 | 1 | |a Nguyen, Tina |e verfasserin |4 aut | |
| 700 | 1 | |a Coig, Rene |e verfasserin |4 aut | |
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