Nanoencapsulation of R-phycoerytrin extracted from Solieria filiformis improves protein stability and enables its biological application as a fluorescent dye
We aimed to encapsulate R-PE to improve its stability for use as a fluorescent probe for cancer cells. Purified R-PE from the algae Solieria filiformis was encapsulated in polymeric nanoparticles using PCL. Nanoparticles were characterised and R-PE release was evaluated. Also, cellular uptake using breast and prostate cancer cells were performed. Nanoparticles presented nanometric particle size (198.8 ± 0.06 nm) with low polydispersity (0.13 ± 0.022), negative zeta potential (-18.7 ± 1.10 mV), and 50.0 ± 7.3% encapsulation. FTIR revealed that R-PE is molecularly dispersed in PCL. DSC peak at 307 °C indicates the presence of R-PE in the nanoparticle. Also, in vitro, it was demonstrated low release for nanoparticles and degradation for the free R-PE. Finally, cellular uptake demonstrated the potential of R-PE/PCL nanoparticles for cancer cell detection. Nanoparticles loaded with R-PE can overcome instability and allow application as a fluorescent probe for cancer cells.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:40 |
|---|---|
| Enthalten in: |
Journal of microencapsulation - 40(2023), 1 vom: 11. Jan., Seite 37-52 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Pereira Martins, Jéssica Roberta [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Fluorescent Dyes |
|---|
| Anmerkungen: |
Date Completed 23.02.2023 Date Revised 23.02.2023 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1080/02652048.2023.2168081 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM351412638 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM351412638 | ||
| 003 | DE-627 | ||
| 005 | 20231226051314.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1080/02652048.2023.2168081 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1171.xml |
| 035 | |a (DE-627)NLM351412638 | ||
| 035 | |a (NLM)36630267 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Pereira Martins, Jéssica Roberta |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Nanoencapsulation of R-phycoerytrin extracted from Solieria filiformis improves protein stability and enables its biological application as a fluorescent dye |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 23.02.2023 | ||
| 500 | |a Date Revised 23.02.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a We aimed to encapsulate R-PE to improve its stability for use as a fluorescent probe for cancer cells. Purified R-PE from the algae Solieria filiformis was encapsulated in polymeric nanoparticles using PCL. Nanoparticles were characterised and R-PE release was evaluated. Also, cellular uptake using breast and prostate cancer cells were performed. Nanoparticles presented nanometric particle size (198.8 ± 0.06 nm) with low polydispersity (0.13 ± 0.022), negative zeta potential (-18.7 ± 1.10 mV), and 50.0 ± 7.3% encapsulation. FTIR revealed that R-PE is molecularly dispersed in PCL. DSC peak at 307 °C indicates the presence of R-PE in the nanoparticle. Also, in vitro, it was demonstrated low release for nanoparticles and degradation for the free R-PE. Finally, cellular uptake demonstrated the potential of R-PE/PCL nanoparticles for cancer cell detection. Nanoparticles loaded with R-PE can overcome instability and allow application as a fluorescent probe for cancer cells | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Nanocapsules | |
| 650 | 4 | |a R-phycoerythrin | |
| 650 | 4 | |a fluorescent probe | |
| 650 | 4 | |a polycaprolactone | |
| 650 | 4 | |a protein | |
| 650 | 7 | |a Fluorescent Dyes |2 NLM | |
| 650 | 7 | |a Polymers |2 NLM | |
| 650 | 7 | |a Polyesters |2 NLM | |
| 700 | 1 | |a Linhares de Aguiar, Antonia Livânia |e verfasserin |4 aut | |
| 700 | 1 | |a Barros Nogueira, Karina Alexandre |e verfasserin |4 aut | |
| 700 | 1 | |a Uchôa Bastos Filho, Acrisio José |e verfasserin |4 aut | |
| 700 | 1 | |a da Silva Moreira, Thais |e verfasserin |4 aut | |
| 700 | 1 | |a Lima Holanda Araújo, Márjory |e verfasserin |4 aut | |
| 700 | 1 | |a Pessoa, Claudia |e verfasserin |4 aut | |
| 700 | 1 | |a Eloy, Josimar O |e verfasserin |4 aut | |
| 700 | 1 | |a da Silva Junior, Ivanildo José |e verfasserin |4 aut | |
| 700 | 1 | |a Petrilli, Raquel |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of microencapsulation |d 1989 |g 40(2023), 1 vom: 11. Jan., Seite 37-52 |w (DE-627)NLM013098217 |x 1464-5246 |7 nnns |
| 773 | 1 | 8 | |g volume:40 |g year:2023 |g number:1 |g day:11 |g month:01 |g pages:37-52 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1080/02652048.2023.2168081 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 40 |j 2023 |e 1 |b 11 |c 01 |h 37-52 | ||