Antinociceptive activity of doliroside B
CONTEXT: Dolichos trilobus Linn (Leguminosae) is often used in Yi ethnic medicine to treat pain, fracture, and rheumatism.
OBJECTIVE: To explore the therapeutic potential of doliroside B (DB) from D. trilobus and its disodium salt (DBDS) and the underlying mechanism in pain.
MATERIALS AND METHODS: In the writhing test, Kunming mice were orally treated with DB and DBDS at doses of 0.31, 0.62, 1.25, 2.5, and 5 mg/kg. Vehicle, morphine, indomethacin, and acetylsalicylic acid were used as negative and positive control on the nociception-induced models, respectively. In the hot plate test, mice were orally treated with DB and DBDS at doses of 2.5, 5, 10, and 20 mg/kg. In the formalin test, mice were orally treated with DB and DBDS at doses of 2.5, 5, 10, and 20 mg/kg. In the meanwhile, lipopolysaccharide-induced inflammatory model in RAW264.7 macrophages was adopted to study the mechanism of pain alleviation for DBDS.
RESULTS: DBDS (5 mg/kg) inhibited the writhing number by 80.2%, which exhibited the highest antinociceptive activity in pain models. DBDS could selectively inhibite the activity of COX-1. Meanwhile, it also reduced the production of NO, iNOS, and IL-6 by 55.8%, 69.0%, and 49.9% inhibition, respectively. It was found that DBDS also positively modulated the function of GABAA1 receptor.
DISCUSSION AND CONCLUSIONS: DBDS displayed antinociceptive activity by acting on both the peripheral and central nervous systems, which may act on multitargets. Further work is warranted for developing DBDS into a potential drug for the treatment of pain.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:61 |
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| Enthalten in: |
Pharmaceutical biology - 61(2023), 1 vom: 10. Dez., Seite 201-212 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Bai, Xishan [VerfasserIn] |
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| Links: |
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| Themen: |
Analgesic mechanism |
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| Anmerkungen: |
Date Completed 13.01.2023 Date Revised 20.01.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.1080/13880209.2022.2163407 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM351394974 |
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| 100 | 1 | |a Bai, Xishan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Antinociceptive activity of doliroside B |
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| 500 | |a Date Revised 20.01.2023 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a CONTEXT: Dolichos trilobus Linn (Leguminosae) is often used in Yi ethnic medicine to treat pain, fracture, and rheumatism | ||
| 520 | |a OBJECTIVE: To explore the therapeutic potential of doliroside B (DB) from D. trilobus and its disodium salt (DBDS) and the underlying mechanism in pain | ||
| 520 | |a MATERIALS AND METHODS: In the writhing test, Kunming mice were orally treated with DB and DBDS at doses of 0.31, 0.62, 1.25, 2.5, and 5 mg/kg. Vehicle, morphine, indomethacin, and acetylsalicylic acid were used as negative and positive control on the nociception-induced models, respectively. In the hot plate test, mice were orally treated with DB and DBDS at doses of 2.5, 5, 10, and 20 mg/kg. In the formalin test, mice were orally treated with DB and DBDS at doses of 2.5, 5, 10, and 20 mg/kg. In the meanwhile, lipopolysaccharide-induced inflammatory model in RAW264.7 macrophages was adopted to study the mechanism of pain alleviation for DBDS | ||
| 520 | |a RESULTS: DBDS (5 mg/kg) inhibited the writhing number by 80.2%, which exhibited the highest antinociceptive activity in pain models. DBDS could selectively inhibite the activity of COX-1. Meanwhile, it also reduced the production of NO, iNOS, and IL-6 by 55.8%, 69.0%, and 49.9% inhibition, respectively. It was found that DBDS also positively modulated the function of GABAA1 receptor | ||
| 520 | |a DISCUSSION AND CONCLUSIONS: DBDS displayed antinociceptive activity by acting on both the peripheral and central nervous systems, which may act on multitargets. Further work is warranted for developing DBDS into a potential drug for the treatment of pain | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Analgesic mechanism | |
| 650 | 4 | |a COX-1 | |
| 650 | 4 | |a GABAA1 receptor | |
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| 700 | 1 | |a Li, Yanhong |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Yuxiao |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Min |e verfasserin |4 aut | |
| 700 | 1 | |a Luo, Ming |e verfasserin |4 aut | |
| 700 | 1 | |a Tian, Kai |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Mengyuan |e verfasserin |4 aut | |
| 700 | 1 | |a Xiong, Yong |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Ya |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Yukui |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Haibo |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Xiangzhong |e verfasserin |4 aut | |
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