The Strange Case : The Unsymmetric Cisplatin-Based Pt(IV) Prodrug [Pt(CH3COO)Cl2(NH3)2(OH)] Exhibits Higher Cytotoxic Activity with respect to Its Symmetric Congeners due to Carrier-Mediated Cellular Uptake

Copyright © 2022 Elisabetta Gabano et al..

The biological behavior of the axially unsymmetric antitumor prodrug (OC-6-44)-acetatodiamminedichloridohydroxidoplatinum(IV), 2, was deeply investigated and compared with that of analogous symmetric Pt(IV) complexes, namely, dihydroxido 1 and diacetato 3, which have a similar structure. The complexes were tested on a panel of human tumor cell lines. Complex 2 showed an anomalous higher cytotoxicity (similar to that of cisplatin) with respect to their analogues 1 and 3. Their reduction potentials, reduction kinetics, lipophilicity, and membrane affinity are compared. Cellular uptake and DNA platination of Pt(IV) complexes were deeply investigated in the sensitive A2780 human ovarian cancer cell line and in the corresponding resistant A2780cisR subline. The unexpected activity of 2 appears to be related to its peculiar cellular accumulation and not to a different rate of reduction or a different efficacy in DNA platination and/or efficiency in apoptosis induction. Although the exact mechanism of cell uptake is not fully deciphered, a series of naïve experiments indicates an energy-dependent, carrier-mediated transport: the organic cation transporters (OCTs) are the likely proteins involved.

Medienart:

E-Artikel

Erscheinungsjahr:

2022

Erschienen:

2022

Enthalten in:

Zur Gesamtaufnahme - volume:2022

Enthalten in:

Bioinorganic chemistry and applications - 2022(2022) vom: 03., Seite 3698391

Sprache:

Englisch

Beteiligte Personen:

Gabano, Elisabetta [VerfasserIn]
Zanellato, Ilaria [VerfasserIn]
Pinton, Giulia [VerfasserIn]
Moro, Laura [VerfasserIn]
Ravera, Mauro [VerfasserIn]
Osella, Domenico [VerfasserIn]

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Date Revised 11.01.2023

published: Electronic-eCollection

Citation Status PubMed-not-MEDLINE

doi:

10.1155/2022/3698391

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM351314385