The enrichment characterisation of Nitrospira under high DO conditions
Nitrite-oxidizing bacteria (NOB) are crucial to nitrification and nitrogen elimination in wastewater treatment. Mass reports exist on the links between NOB and other microorganisms, for instance, ammonia-oxidizing bacteria (AOB). However, a few studies exist on the enrichment characterisation of NOB under high dissolved oxygen (DO) conditions. In this study, NOB was designed to be enriched individually under high DO conditions in a continuous aeration sequencing batch reactor (SBR), and the kinetic characterisation of NOB was evaluated. The analysis revealed that the average NO2--N removal rate was steady above 98%, with DO and NO2--N being 3-5 mg L-1 and 50-450 mg L-1, respectively. The NO2--N removal efficiency of the system was significantly enhanced and better than in other studies. The high-throughput sequencing suggested that Parcubacteria_ genera_incertae_sedis was the first dominant genus (21.99%), which often appeared in the NOB biological community with Nitrospira. However, the dominant genus NOB was Nitrospira rather than Nitrobacter (8.49%). This result suggested that Nitrospira was capable of higher NO2--N removal. But lower relative abundance indicated that excessive NO2--N had an adverse effect on the enrichment and activity of Nitrospira. In addition, the nitrite half-saturation constant (KNO2) and the oxygen half-saturation constant (KO) were 1.71 ± 0.19 mg L-1 and 0.95 ± 0.10 mg L-1, respectively. These results showed that the enriched Nitrospira bacteria had different characteristics at the strain level, which can be used as a theoretical basis for wastewater treatment plant design and optimisation.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:45 |
|---|---|
| Enthalten in: |
Environmental technology - 45(2024), 11 vom: 23. Apr., Seite 2156-2170 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Fu, Kunming [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
7664-41-7 |
|---|
| Anmerkungen: |
Date Completed 15.04.2024 Date Revised 15.04.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1080/09593330.2023.2165457 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM351130721 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM351130721 | ||
| 003 | DE-627 | ||
| 005 | 20240415232826.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1080/09593330.2023.2165457 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1376.xml |
| 035 | |a (DE-627)NLM351130721 | ||
| 035 | |a (NLM)36601901 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Fu, Kunming |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The enrichment characterisation of Nitrospira under high DO conditions |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 15.04.2024 | ||
| 500 | |a Date Revised 15.04.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Nitrite-oxidizing bacteria (NOB) are crucial to nitrification and nitrogen elimination in wastewater treatment. Mass reports exist on the links between NOB and other microorganisms, for instance, ammonia-oxidizing bacteria (AOB). However, a few studies exist on the enrichment characterisation of NOB under high dissolved oxygen (DO) conditions. In this study, NOB was designed to be enriched individually under high DO conditions in a continuous aeration sequencing batch reactor (SBR), and the kinetic characterisation of NOB was evaluated. The analysis revealed that the average NO2--N removal rate was steady above 98%, with DO and NO2--N being 3-5 mg L-1 and 50-450 mg L-1, respectively. The NO2--N removal efficiency of the system was significantly enhanced and better than in other studies. The high-throughput sequencing suggested that Parcubacteria_ genera_incertae_sedis was the first dominant genus (21.99%), which often appeared in the NOB biological community with Nitrospira. However, the dominant genus NOB was Nitrospira rather than Nitrobacter (8.49%). This result suggested that Nitrospira was capable of higher NO2--N removal. But lower relative abundance indicated that excessive NO2--N had an adverse effect on the enrichment and activity of Nitrospira. In addition, the nitrite half-saturation constant (KNO2) and the oxygen half-saturation constant (KO) were 1.71 ± 0.19 mg L-1 and 0.95 ± 0.10 mg L-1, respectively. These results showed that the enriched Nitrospira bacteria had different characteristics at the strain level, which can be used as a theoretical basis for wastewater treatment plant design and optimisation | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Nitrospira | |
| 650 | 4 | |a enrichment | |
| 650 | 4 | |a high DO | |
| 650 | 4 | |a kinetic characterisation | |
| 650 | 4 | |a nitrite-oxidizing bacteria | |
| 650 | 7 | |a Nitrites |2 NLM | |
| 650 | 7 | |a Nitrogen Dioxide |2 NLM | |
| 650 | 7 | |a S7G510RUBH |2 NLM | |
| 650 | 7 | |a Ammonia |2 NLM | |
| 650 | 7 | |a 7664-41-7 |2 NLM | |
| 700 | 1 | |a Zhang, Xuemeng |e verfasserin |4 aut | |
| 700 | 1 | |a Fan, Yang |e verfasserin |4 aut | |
| 700 | 1 | |a Bian, Yihao |e verfasserin |4 aut | |
| 700 | 1 | |a Qiu, Fuguo |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Xiuqin |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Environmental technology |d 1993 |g 45(2024), 11 vom: 23. Apr., Seite 2156-2170 |w (DE-627)NLM098202545 |x 1479-487X |7 nnns |
| 773 | 1 | 8 | |g volume:45 |g year:2024 |g number:11 |g day:23 |g month:04 |g pages:2156-2170 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1080/09593330.2023.2165457 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 45 |j 2024 |e 11 |b 23 |c 04 |h 2156-2170 | ||