Room-temperature serial synchrotron crystallography of the human phosphatase PTP1B
open access..
Room-temperature X-ray crystallography provides unique insights into protein conformational heterogeneity, but obtaining sufficiently large protein crystals is a common hurdle. Serial synchrotron crystallography (SSX) helps to address this hurdle by allowing the use of many medium- to small-sized crystals. Here, a recently introduced serial sample-support chip system has been used to obtain the first SSX structure of a human phosphatase, specifically protein tyrosine phosphatase 1B (PTP1B) in the unliganded (apo) state. In previous apo room-temperature structures, the active site and allosteric sites adopted alternate conformations, including open and closed conformations of the active-site WPD loop and of a distal allosteric site. By contrast, in our SSX structure the active site is best fitted with a single conformation, but the distal allosteric site is best fitted with alternate conformations. This observation argues for additional nuance in interpreting the nature of allosteric coupling in this protein. Overall, our results illustrate the promise of serial methods for room-temperature crystallography, as well as future avant-garde crystallography experiments, for PTP1B and other proteins.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:79 |
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| Enthalten in: |
Acta crystallographica. Section F, Structural biology communications - 79(2023), Pt 1 vom: 01. Jan., Seite 23-30 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Sharma, Shivani [VerfasserIn] |
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| Links: |
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| Themen: |
Allostery |
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| Anmerkungen: |
Date Completed 06.01.2023 Date Revised 09.07.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1107/S2053230X22011645 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM351095551 |
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| 520 | |a Room-temperature X-ray crystallography provides unique insights into protein conformational heterogeneity, but obtaining sufficiently large protein crystals is a common hurdle. Serial synchrotron crystallography (SSX) helps to address this hurdle by allowing the use of many medium- to small-sized crystals. Here, a recently introduced serial sample-support chip system has been used to obtain the first SSX structure of a human phosphatase, specifically protein tyrosine phosphatase 1B (PTP1B) in the unliganded (apo) state. In previous apo room-temperature structures, the active site and allosteric sites adopted alternate conformations, including open and closed conformations of the active-site WPD loop and of a distal allosteric site. By contrast, in our SSX structure the active site is best fitted with a single conformation, but the distal allosteric site is best fitted with alternate conformations. This observation argues for additional nuance in interpreting the nature of allosteric coupling in this protein. Overall, our results illustrate the promise of serial methods for room-temperature crystallography, as well as future avant-garde crystallography experiments, for PTP1B and other proteins | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a X-ray crystallography | |
| 650 | 4 | |a allostery | |
| 650 | 4 | |a apo protein tyrosine phosphatase 1B | |
| 650 | 4 | |a phosphatases | |
| 650 | 4 | |a room-temperature serial crystallography | |
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| 700 | 1 | |a Ebrahim, Ali |e verfasserin |4 aut | |
| 700 | 1 | |a Keedy, Daniel A |e verfasserin |4 aut | |
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