Genetic and Phenotypic Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Isolates from a Tertiary Hospital in Beijing
© 2022 Ni et al..
Objective: Klebsiella pneumoniae is a common multidrug-resistant pathogen that jeopardizes the health of hospitalized patients. We aimed to study the phenotypic and genotypic characteristics of carbapenem-resistant K. pneumoniae (CRKP) isolates from a hospital in Beijing.
Methods: Twenty-four CRKP clinical isolates were collected within a half-year to investigate antimicrobial resistance and genomic characteristics. Illumina and Nanopore sequencing were performed to assemble and annotate genomes.
Results: All strains were multi-drug resistant. Twenty-two strains carried the bla KPC-2 gene and two harbored bla NDM-5. Multilocus sequence type(MLST) analysis identified five sequence types; most isolates belonged to ST11. Three strains were isolated from the same patient; each carried a different plasmid replicon, either IncFII (pHN7A8), IncX, or IncFIB (K).
Conclusion: This study furthers the understanding of CRKP antimicrobial resistance genotypes, and may facilitate the control of nosocomial infections caused by antimicrobial-resistant pathogens.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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| Enthalten in: |
Infection and drug resistance - 15(2022) vom: 01., Seite 7503-7508 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ni, Qin [VerfasserIn] |
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| Links: |
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| Themen: |
BlaKPC-2 |
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| Anmerkungen: |
Date Revised 27.12.2022 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.2147/IDR.S395920 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM350822204 |
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| 245 | 1 | 0 | |a Genetic and Phenotypic Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Isolates from a Tertiary Hospital in Beijing |
| 264 | 1 | |c 2022 | |
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| 500 | |a Date Revised 27.12.2022 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © 2022 Ni et al. | ||
| 520 | |a Objective: Klebsiella pneumoniae is a common multidrug-resistant pathogen that jeopardizes the health of hospitalized patients. We aimed to study the phenotypic and genotypic characteristics of carbapenem-resistant K. pneumoniae (CRKP) isolates from a hospital in Beijing | ||
| 520 | |a Methods: Twenty-four CRKP clinical isolates were collected within a half-year to investigate antimicrobial resistance and genomic characteristics. Illumina and Nanopore sequencing were performed to assemble and annotate genomes | ||
| 520 | |a Results: All strains were multi-drug resistant. Twenty-two strains carried the bla KPC-2 gene and two harbored bla NDM-5. Multilocus sequence type(MLST) analysis identified five sequence types; most isolates belonged to ST11. Three strains were isolated from the same patient; each carried a different plasmid replicon, either IncFII (pHN7A8), IncX, or IncFIB (K) | ||
| 520 | |a Conclusion: This study furthers the understanding of CRKP antimicrobial resistance genotypes, and may facilitate the control of nosocomial infections caused by antimicrobial-resistant pathogens | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Klebsiella pneumoniae | |
| 650 | 4 | |a ST11 | |
| 650 | 4 | |a blaKPC-2 | |
| 650 | 4 | |a carbapenemase | |
| 650 | 4 | |a drug resistance | |
| 700 | 1 | |a Yao, Xingwei |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jinhui |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Jinghan |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Kaiying |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Xiong |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Peihan |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Lang |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Peng |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Shenlong |e verfasserin |4 aut | |
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