Evaluation of Newcastle disease virus LaSota strain attenuated by codon pair deoptimization of the HN and F genes for in ovo vaccination
Published by Elsevier B.V..
In ovo vaccination is an attractive immunization approach for the poultry industry. However, commonly used Newcastle disease virus (NDV) vaccines cannot be administered in ovo because of the reduced hatchability and embryo mortality. The codon pair deoptimization (CPD) approach has been used to efficiently and rapidly attenuate viruses by targeting the virulence genes. In this study, we aimed to attenuate the NDV LaSota (LS) vaccine strain for in ovo vaccination by CPD of the fusion (F) or/and hemagglutinin-neuraminidase (HN) genes with approximately 44 % suboptimal codon substitutions. Three NDV LS recombinants expressing codon deoptimized F (rLS/F-d), HN (rLS/HN-d), or both genes (rLS/F+HN-d) were generated using reverse genetics technology. Biological assays showed that the CPD viruses retained similar hemagglutination activity and growth ability to the parental rLS virus. The CPD of the HN gene slightly attenuated the rLS/HN-d and rLS/F+HN-d viruses, whereas the CPD of the F gene marginally increased the rLS/F-d virus pathogenicity compared to rLS. Nevertheless, all three CPD rLS viruses were still lethal to 10-day-old specific-pathogen-free (SPF) chicken embryos. In ovo inoculation of 18-day-old SPF chicken embryos with the CPD viruses severely reduced chicken's hatch and survival rates. These results suggested that the CPD of the surface glycoprotein genes of the LS strain at the current level of suboptimal codon substitutions could not sufficiently attenuate the virus for use as an in ovo vaccine, and codon deoptimizing a greater proportion of the F and HN genes or additional gene(s) may be required for sufficient attenuation of the LS strain.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:277 |
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| Enthalten in: |
Veterinary microbiology - 277(2023) vom: 13. Feb., Seite 109625 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Eldemery, Fatma [VerfasserIn] |
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| Links: |
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| Themen: |
Attenuation |
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| Anmerkungen: |
Date Completed 10.01.2023 Date Revised 11.01.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.vetmic.2022.109625 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Published by Elsevier B.V. | ||
| 520 | |a In ovo vaccination is an attractive immunization approach for the poultry industry. However, commonly used Newcastle disease virus (NDV) vaccines cannot be administered in ovo because of the reduced hatchability and embryo mortality. The codon pair deoptimization (CPD) approach has been used to efficiently and rapidly attenuate viruses by targeting the virulence genes. In this study, we aimed to attenuate the NDV LaSota (LS) vaccine strain for in ovo vaccination by CPD of the fusion (F) or/and hemagglutinin-neuraminidase (HN) genes with approximately 44 % suboptimal codon substitutions. Three NDV LS recombinants expressing codon deoptimized F (rLS/F-d), HN (rLS/HN-d), or both genes (rLS/F+HN-d) were generated using reverse genetics technology. Biological assays showed that the CPD viruses retained similar hemagglutination activity and growth ability to the parental rLS virus. The CPD of the HN gene slightly attenuated the rLS/HN-d and rLS/F+HN-d viruses, whereas the CPD of the F gene marginally increased the rLS/F-d virus pathogenicity compared to rLS. Nevertheless, all three CPD rLS viruses were still lethal to 10-day-old specific-pathogen-free (SPF) chicken embryos. In ovo inoculation of 18-day-old SPF chicken embryos with the CPD viruses severely reduced chicken's hatch and survival rates. These results suggested that the CPD of the surface glycoprotein genes of the LS strain at the current level of suboptimal codon substitutions could not sufficiently attenuate the virus for use as an in ovo vaccine, and codon deoptimizing a greater proportion of the F and HN genes or additional gene(s) may be required for sufficient attenuation of the LS strain | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Attenuation | |
| 650 | 4 | |a Chicken embryo | |
| 650 | 4 | |a Codon pair deoptimization | |
| 650 | 4 | |a In ovo vaccination | |
| 650 | 4 | |a LaSota vaccine | |
| 650 | 4 | |a Newcastle disease virus | |
| 650 | 4 | |a Reverse genetics | |
| 650 | 7 | |a Viral Vaccines |2 NLM | |
| 650 | 7 | |a HN Protein |2 NLM | |
| 650 | 7 | |a Codon |2 NLM | |
| 700 | 1 | |a Ou, Changbo |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Taejoong |e verfasserin |4 aut | |
| 700 | 1 | |a Spatz, Stephen |e verfasserin |4 aut | |
| 700 | 1 | |a Dunn, John |e verfasserin |4 aut | |
| 700 | 1 | |a Silva, Robert |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Qingzhong |e verfasserin |4 aut | |
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