The Aging Process : A Metabolomics Perspective
Aging process is characterized by a progressive decline of several organic, physiological, and metabolic functions whose precise mechanism remains unclear. Metabolomics allows the identification of several metabolites and may contribute to clarifying the aging-regulated metabolic pathways. We aimed to investigate aging-related serum metabolic changes using a metabolomics approach. Fasting blood serum samples from 138 apparently healthy individuals (20−70 years old, 56% men) were analyzed by Proton Nuclear Magnetic Resonance spectroscopy (1H NMR) and Liquid Chromatography-High-Resolution Mass Spectrometry (LC-HRMS), and for clinical markers. Associations of the metabolic profile with age were explored via Correlations (r); Metabolite Set Enrichment Analysis; Multiple Linear Regression; and Aging Metabolism Breakpoint. The age increase was positively correlated (0.212 ≤ r ≤ 0.370, p < 0.05) with the clinical markers (total cholesterol, HDL, LDL, VLDL, triacylglyceride, and glucose levels); negatively correlated (−0.285 ≤ r ≤ −0.214, p < 0.05) with tryptophan, 3-hydroxyisobutyrate, asparagine, isoleucine, leucine, and valine levels, but positively (0.237 ≤ r ≤ 0.269, p < 0.05) with aspartate and ornithine levels. These metabolites resulted in three enriched pathways: valine, leucine, and isoleucine degradation, urea cycle, and ammonia recycling. Additionally, serum metabolic levels of 3-hydroxyisobutyrate, isoleucine, aspartate, and ornithine explained 27.3% of the age variation, with the aging metabolism breakpoint occurring after the third decade of life. These results indicate that the aging process is potentially associated with reduced serum branched-chain amino acid levels (especially after the third decade of life) and progressively increased levels of serum metabolites indicative of the urea cycle.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:27 |
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| Enthalten in: |
Molecules (Basel, Switzerland) - 27(2022), 24 vom: 07. Dez. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Castro, Alex [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 26.12.2022 Date Revised 08.03.2023 published: Electronic Citation Status MEDLINE |
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| doi: |
10.3390/molecules27248656 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 100 | 1 | |a Castro, Alex |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The Aging Process |b A Metabolomics Perspective |
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| 500 | |a Date Revised 08.03.2023 | ||
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| 520 | |a Aging process is characterized by a progressive decline of several organic, physiological, and metabolic functions whose precise mechanism remains unclear. Metabolomics allows the identification of several metabolites and may contribute to clarifying the aging-regulated metabolic pathways. We aimed to investigate aging-related serum metabolic changes using a metabolomics approach. Fasting blood serum samples from 138 apparently healthy individuals (20−70 years old, 56% men) were analyzed by Proton Nuclear Magnetic Resonance spectroscopy (1H NMR) and Liquid Chromatography-High-Resolution Mass Spectrometry (LC-HRMS), and for clinical markers. Associations of the metabolic profile with age were explored via Correlations (r); Metabolite Set Enrichment Analysis; Multiple Linear Regression; and Aging Metabolism Breakpoint. The age increase was positively correlated (0.212 ≤ r ≤ 0.370, p < 0.05) with the clinical markers (total cholesterol, HDL, LDL, VLDL, triacylglyceride, and glucose levels); negatively correlated (−0.285 ≤ r ≤ −0.214, p < 0.05) with tryptophan, 3-hydroxyisobutyrate, asparagine, isoleucine, leucine, and valine levels, but positively (0.237 ≤ r ≤ 0.269, p < 0.05) with aspartate and ornithine levels. These metabolites resulted in three enriched pathways: valine, leucine, and isoleucine degradation, urea cycle, and ammonia recycling. Additionally, serum metabolic levels of 3-hydroxyisobutyrate, isoleucine, aspartate, and ornithine explained 27.3% of the age variation, with the aging metabolism breakpoint occurring after the third decade of life. These results indicate that the aging process is potentially associated with reduced serum branched-chain amino acid levels (especially after the third decade of life) and progressively increased levels of serum metabolites indicative of the urea cycle | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Signini, Étore F |e verfasserin |4 aut | |
| 700 | 1 | |a De Oliveira, Juliana Magalhães |e verfasserin |4 aut | |
| 700 | 1 | |a Di Medeiros Leal, Maria Carolina Bezerra |e verfasserin |4 aut | |
| 700 | 1 | |a Rehder-Santos, Patrícia |e verfasserin |4 aut | |
| 700 | 1 | |a Millan-Mattos, Juliana C |e verfasserin |4 aut | |
| 700 | 1 | |a Minatel, Vinicius |e verfasserin |4 aut | |
| 700 | 1 | |a Pantoni, Camila B F |e verfasserin |4 aut | |
| 700 | 1 | |a Oliveira, Regina V |e verfasserin |4 aut | |
| 700 | 1 | |a Catai, Aparecida M |e verfasserin |4 aut | |
| 700 | 1 | |a Ferreira, Antônio G |e verfasserin |4 aut | |
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