Ultrasensitive Determination of Glial-Fibrillary-Acidic-Protein (GFAP) in Human Serum-Matrix with a Label-Free Impedimetric Immunosensor
In this work, immobilizing anti-GFAP antibodies via covalent attachment onto L-cysteine/gold nanoparticles that were modified with screen-printed carbon electrodes (Anti-GFAP/L-cys/AuNps/SPCE) resulted in the development of a sensitive label-free impedance immunosensor for the detection of Glial Fibrillary Acidic Protein (GFAP). The immunosensor's stepwise construction was studied using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). L-cysteine was chosen as the linker between GFAP antibodies and Au NPs/SPCE because it enables the guided and stable immobilization of GFAP antibodies, thus resulting in increased immunosensor sensitivity. As a redox probe, 5 mM of [Fe(CN)6]3-/4- was used to measure the electron-transfer resistance (Ret), which was raised by the binding of antigens to the immobilized anti-GFAP on the surface of the modified electrode. A linear correlation between Rct and GFAP concentration was achieved under optimum conditions in the range of 1.0-1000.0 pg/mL, with an extraordinarily low detection limit of 51.0 fg/mL. The suggested immunosensor was successfully used to detect the presence of GFAP in human blood serum samples, yielding good findings. As a result, the proposed platform may be utilized to monitor central nervous system injuries.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
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| Enthalten in: |
Biosensors - 12(2022), 12 vom: 14. Dez. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ozcelikay, Goksu [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 26.12.2022 Date Revised 26.12.2022 published: Electronic Citation Status MEDLINE |
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| doi: |
10.3390/bios12121165 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM350626545 |
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| 500 | |a published: Electronic | ||
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| 520 | |a In this work, immobilizing anti-GFAP antibodies via covalent attachment onto L-cysteine/gold nanoparticles that were modified with screen-printed carbon electrodes (Anti-GFAP/L-cys/AuNps/SPCE) resulted in the development of a sensitive label-free impedance immunosensor for the detection of Glial Fibrillary Acidic Protein (GFAP). The immunosensor's stepwise construction was studied using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). L-cysteine was chosen as the linker between GFAP antibodies and Au NPs/SPCE because it enables the guided and stable immobilization of GFAP antibodies, thus resulting in increased immunosensor sensitivity. As a redox probe, 5 mM of [Fe(CN)6]3-/4- was used to measure the electron-transfer resistance (Ret), which was raised by the binding of antigens to the immobilized anti-GFAP on the surface of the modified electrode. A linear correlation between Rct and GFAP concentration was achieved under optimum conditions in the range of 1.0-1000.0 pg/mL, with an extraordinarily low detection limit of 51.0 fg/mL. The suggested immunosensor was successfully used to detect the presence of GFAP in human blood serum samples, yielding good findings. As a result, the proposed platform may be utilized to monitor central nervous system injuries | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Au nanoparticles (Au NPs) | |
| 650 | 4 | |a glial fibrillary acidic protein (GFAP) | |
| 650 | 4 | |a label-free impedance immunosensor | |
| 650 | 4 | |a screen printed carbon electrode (SPCE) | |
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| 700 | 1 | |a Mollarasouli, Fariba |e verfasserin |4 aut | |
| 700 | 1 | |a Unal, Mehmet Altay |e verfasserin |4 aut | |
| 700 | 1 | |a Gucuyener, Kıvılcım |e verfasserin |4 aut | |
| 700 | 1 | |a Ozkan, Sibel A |e verfasserin |4 aut | |
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