Correlation analysis of age and microbial characteristics in saliva and feces of high-risk population of upper gastrointestinal cancer

Objective: To explore the correlation between age and diversity and microbial composition in saliva and feces microbiota in high-risk population of upper gastrointestinal cancer. Methods: Based on the national project on early diagnosis and early treatment of upper gastrointestinal cancer, 38 participants were enrolled in Linzhou in Henan province in August 2019. The participant information was collected by questionnaire. Saliva and feces specimens were collected from each participant for 16S rRNA sequencing and bioinformatics analysis. Spearman rank correlation was used to analyze the correlation between age and α diversity (Observed ASVs and Shannon index) and relative abundance of microbiota (phyla, genera, and species) in saliva and feces. Results: The median age (age range) of 38 participants was 54 (43-60) years old, and there were 16 males (42.1%). The Observed ASVs of saliva was negatively correlated with age (rs=-0.35, P<0.05), but the observed ASVs of feces was not correlated with age. In saliva, the relative abundance of Treponema (rs=‒0.44, P<0.05), Alloprevotella (rs=‒0.42, P<0.05), and Porphyromonas (rs=‒0.41,P<0.05) were significantly negatively correlated with age. At the species level, the relative abundance of Porphyromonas endodontalis, Alloprevotella tannerae, Haemophilus influenza, Moraxella bovoculi, Prevotella sp.oral clone ID019, and Prevotella sp.oral clone ASCG10 in saliva were significantly negatively correlated with age, and the rs values were -0.50, -0.40, -0.38, -0.35, -0.33 and -0.33 (P<0.05), respectively. In feces, the relative abundance of Enterobacteria (rs=-0.35, P<0.05), Escherichia (rs=-0.33, P<0.05), and Bifidobacteria (rs=0.33, P<0.05) were correlated with age. At the species level, the relative abundance of Romboutsia sedimentorum, Citrobacter murliniae, and bacteroides uniformis in feces were correlated with age, and the rs values were -0.42, -0.37 and 0.36 (P<0.05), respectively. Conclusion: Age of the high-risk population of upper gastrointestinal cancer is correlated with the relative abundance of microbiota in saliva and feces.

Medienart:	E-Artikel

Erscheinungsjahr:	2022
Erschienen:	2022

Enthalten in:	Zur Gesamtaufnahme - volume:56
Enthalten in:	Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine - 56(2022), 12 vom: 06. Dez., Seite 1759-1766

Sprache:	Chinesisch

Beteiligte Personen:	Li, M J [VerfasserIn] Shao, D T [VerfasserIn] Zhou, J C [VerfasserIn] Gu, J H [VerfasserIn] Fan, Z Y [VerfasserIn] Qin, J J [VerfasserIn] Li, X Q [VerfasserIn] Hao, C Q [VerfasserIn] Wei, W W [VerfasserIn]

Links:	Volltext

Themen:	English Abstract Journal Article RNA, Ribosomal, 16S

Anmerkungen:	Date Completed 21.12.2022 Date Revised 22.12.2022 published: Print Citation Status MEDLINE

doi:	10.3760/cma.j.cn112150-20220715-00720

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	NLM350483558