Improved immunity and mean lifespan in mature mice genetically deficient in catecholamine synthesis after living with wild type for two months
Copyright © 2022 SEGG. Publicado por Elsevier España, S.L.U. All rights reserved..
INTRODUCTION: Mice hemizygous in tyrosine hydroxylase (TH-HZ), the limiting enzyme in catecholamine synthesis, show premature immunosenescence, which in females is associated with a shorter lifespan than the corresponding controls (WT). The coexistence of TH-Hz with WT improves the immune function in both males and females in adulthood.
OBJECTIVE: To test whether cohabitation for two months of mature male TH-HZ with WT improves the immune function of the former and whether this impacts the lifespan.
MATERIAL AND METHODS: Mature male ICR-CD1 mice (13 ± 1 months) TH-HZ coexisted with WT (2:4 ratio in each cage) for two months. Peritoneal leukocytes were extracted from all animals at baseline, one month, and two months after cohabitation, and macrophage phagocytic capacity, macrophage and lymphocyte chemotaxis, natural killer (NK) antitumor activity, and lymphoproliferative capacity in response to the mitogens concanavalin A and lipopolysaccharide (LPS) were assessed. The animals were maintained under these conditions until their natural death.
RESULTS: The TH-HZ, which start, in general, with lower values than the WT in the immune functions studied, improved them after two months of cohabitation, becoming similar to those of the controls. This improvement was already observed in NK activity after one month of cohabitation. The TH-HZ presented lower mean longevity than WT, but when they cohabited with WT, it was similar to the latter.
CONCLUSION: The coexistence of TH-HZ male mice with WT mice for two months at mature age improves these genetically modified animals' immune response and longevity.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:58 |
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| Enthalten in: |
Revista espanola de geriatria y gerontologia - 58(2023), 1 vom: 12. Jan., Seite 15-21 |
| Sprache: |
Spanisch |
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| Weiterer Titel: |
Mejoría de la inmunidad y la esperanza de vida en ratones maduros, genéticamente deficientes en la síntesis de catecolaminas, tras convivir con controles durante dos meses |
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| Beteiligte Personen: |
Ceprian, Noemi [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 07.03.2023 Date Revised 07.03.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.regg.2022.11.004 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
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| 245 | 1 | 0 | |a Improved immunity and mean lifespan in mature mice genetically deficient in catecholamine synthesis after living with wild type for two months |
| 246 | 3 | 3 | |a Mejoría de la inmunidad y la esperanza de vida en ratones maduros, genéticamente deficientes en la síntesis de catecolaminas, tras convivir con controles durante dos meses |
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| 500 | |a Date Completed 07.03.2023 | ||
| 500 | |a Date Revised 07.03.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2022 SEGG. Publicado por Elsevier España, S.L.U. All rights reserved. | ||
| 520 | |a INTRODUCTION: Mice hemizygous in tyrosine hydroxylase (TH-HZ), the limiting enzyme in catecholamine synthesis, show premature immunosenescence, which in females is associated with a shorter lifespan than the corresponding controls (WT). The coexistence of TH-Hz with WT improves the immune function in both males and females in adulthood | ||
| 520 | |a OBJECTIVE: To test whether cohabitation for two months of mature male TH-HZ with WT improves the immune function of the former and whether this impacts the lifespan | ||
| 520 | |a MATERIAL AND METHODS: Mature male ICR-CD1 mice (13 ± 1 months) TH-HZ coexisted with WT (2:4 ratio in each cage) for two months. Peritoneal leukocytes were extracted from all animals at baseline, one month, and two months after cohabitation, and macrophage phagocytic capacity, macrophage and lymphocyte chemotaxis, natural killer (NK) antitumor activity, and lymphoproliferative capacity in response to the mitogens concanavalin A and lipopolysaccharide (LPS) were assessed. The animals were maintained under these conditions until their natural death | ||
| 520 | |a RESULTS: The TH-HZ, which start, in general, with lower values than the WT in the immune functions studied, improved them after two months of cohabitation, becoming similar to those of the controls. This improvement was already observed in NK activity after one month of cohabitation. The TH-HZ presented lower mean longevity than WT, but when they cohabited with WT, it was similar to the latter | ||
| 520 | |a CONCLUSION: The coexistence of TH-HZ male mice with WT mice for two months at mature age improves these genetically modified animals' immune response and longevity | ||
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Ambiente social | |
| 650 | 4 | |a Deficiencia en tirosina hidroxilasa | |
| 650 | 4 | |a Deficiency of tyrosine hydroxylase | |
| 650 | 4 | |a Inmunosenescencia prematura | |
| 650 | 4 | |a Longevidad | |
| 650 | 4 | |a Longevity | |
| 650 | 4 | |a Male mice | |
| 650 | 4 | |a Premature immunosenescence | |
| 650 | 4 | |a Ratones macho | |
| 650 | 4 | |a Social environment | |
| 650 | 7 | |a Catecholamines |2 NLM | |
| 650 | 7 | |a Tyrosine 3-Monooxygenase |2 NLM | |
| 650 | 7 | |a EC 1.14.16.2 |2 NLM | |
| 700 | 1 | |a Garrido, Antonio |e verfasserin |4 aut | |
| 700 | 1 | |a De la Fuente, Mónica |e verfasserin |4 aut | |
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