Stable Isotope Probing-RNA Strategy to Study Plant/Fungus Interactions
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature..
The use of stable-isotope probing (SIP) allows tracing specific labeled substrates into fungi leading to a better understanding of their role in biogeochemical cycles and their relationship with their environment. Stable isotope probing combined with ribosomal RNA molecule, conserved in the three kingdoms of life, and messenger RNA analysis permits the linkage of diversity and function. Here, we describe two methods designed to investigate the interactions between plants and their associated mycorrhizal compartment by tracing carbon flux from the host plant to its symbionts.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2605 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2605(2023) vom: 06., Seite 169-186 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Van, Amandine Lê [VerfasserIn] |
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| Links: |
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| Themen: |
63231-63-0 |
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| Anmerkungen: |
Date Completed 19.12.2022 Date Revised 04.03.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-2871-3_9 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a The use of stable-isotope probing (SIP) allows tracing specific labeled substrates into fungi leading to a better understanding of their role in biogeochemical cycles and their relationship with their environment. Stable isotope probing combined with ribosomal RNA molecule, conserved in the three kingdoms of life, and messenger RNA analysis permits the linkage of diversity and function. Here, we describe two methods designed to investigate the interactions between plants and their associated mycorrhizal compartment by tracing carbon flux from the host plant to its symbionts | ||
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