Role of sonic hedgehog ligand in gastric cancer therapeutics
Purpose: The abnormal activation of the sonic hedgehog (SHH) signaling pathway is responsible for the progression of several types of cancers including Gastric Cancer (GC). SHH has been associated with the activation of different signaling pathways. Therefore, in this study, we investigated messenger RNA (mRNA) and protein expression of SHH in gastric malignancies and possible correlation with various clinicopathological parameters.
Materials and Methods: A total of 53 surgically resected tumors and adjacent histologically normal tissues from GC patients were investigated in study subjects. A quantitative real-time polymerase chain reaction and immunohistochemistry methods were used for expression analysis of SHH.
Results: At mRNA level, SHH was overexpressed in 60% (27/45) of GC cases as compared to their adjacent normal tissues. SHH immunohistochemical analysis revealed abundant cytoplasmic localization and overexpression in 43.39% (23/53) of GC tissues. SHH overexpression was not associated with any of the clinicopathological parameters.
Conclusion: Our results showed that SHH is dysregulated in GC and might be considered as a biomarker for GC progression and can be used as a target in cancer therapeutics.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:18 |
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| Enthalten in: |
Journal of cancer research and therapeutics - 18(2022), Supplement vom: 13. Dez., Seite S267-S272 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Akhtar, Kulsum [VerfasserIn] |
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| Links: |
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| Themen: |
Gastric cancer |
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| Anmerkungen: |
Date Completed 29.12.2022 Date Revised 29.12.2022 published: Print Citation Status MEDLINE |
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| doi: |
10.4103/jcrt.jcrt_739_21 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM350230676 |
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| 500 | |a Date Revised 29.12.2022 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Purpose: The abnormal activation of the sonic hedgehog (SHH) signaling pathway is responsible for the progression of several types of cancers including Gastric Cancer (GC). SHH has been associated with the activation of different signaling pathways. Therefore, in this study, we investigated messenger RNA (mRNA) and protein expression of SHH in gastric malignancies and possible correlation with various clinicopathological parameters | ||
| 520 | |a Materials and Methods: A total of 53 surgically resected tumors and adjacent histologically normal tissues from GC patients were investigated in study subjects. A quantitative real-time polymerase chain reaction and immunohistochemistry methods were used for expression analysis of SHH | ||
| 520 | |a Results: At mRNA level, SHH was overexpressed in 60% (27/45) of GC cases as compared to their adjacent normal tissues. SHH immunohistochemical analysis revealed abundant cytoplasmic localization and overexpression in 43.39% (23/53) of GC tissues. SHH overexpression was not associated with any of the clinicopathological parameters | ||
| 520 | |a Conclusion: Our results showed that SHH is dysregulated in GC and might be considered as a biomarker for GC progression and can be used as a target in cancer therapeutics | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Gastric cancer | |
| 650 | 4 | |a immunohistochemistry | |
| 650 | 4 | |a quantitative real-time polymerase chain reaction | |
| 650 | 4 | |a sonic hedgehog | |
| 650 | 7 | |a Hedgehog Proteins |2 NLM | |
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| 650 | 7 | |a SHH protein, human |2 NLM | |
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| 700 | 1 | |a Bhat, Gulzar Ahmad |e verfasserin |4 aut | |
| 700 | 1 | |a Bhat, Ishrat Parveiz |e verfasserin |4 aut | |
| 700 | 1 | |a Syed, Douhath Yousuf |e verfasserin |4 aut | |
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| 700 | 1 | |a Syed, Mudassar |e verfasserin |4 aut | |
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