Defective determination of synthetic cathinones in blood for forensic investigation
Copyright © 2022 Elsevier B.V. All rights reserved..
Antemortem specimens are sometimes the sole sources available for forensic investigation, and samples collected in nonideal ways are inevitably employed to achieve toxicological analysis. It is essential to assess the effects of blood collection tubes on the recoveries of emerging synthetic cathinones (SC) to estimate actual drug concentrations, and no such systematic investigations have been previously carried out. Seventy-one SC with various LogP values were employed to examine commonly used blood collection tubes, including plasma tubes, serum tubes and gel-containing tubes in recoveries which determined by a reliable LC-MS/MS method. Significantly poor recoveries for hydrophobic SC were obtained using serum separating tubes (SST). Notably, the suppressed recoveries in SST can be reversed by adding anticoagulants. Adding a procoagulant to a plasma separating tube (PST) considerably reduced recoveries, which indicated that clotting processes in the presence of polymeric gels contributed to poor recoveries of these hydrophobic drugs. In this study, we find that clotting formation in the presence of polymeric gels could significantly affect the determination of hydrophobic drugs. However, in real-world scenarios, nonideal collection methods are inevitably employed for antemortem specimens. Thus, it is important to rigorously interpret forensic toxicological results, especially for susceptible species.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:539 |
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| Enthalten in: |
Clinica chimica acta; international journal of clinical chemistry - 539(2023) vom: 15. Jan., Seite 122-129 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chen, Ju-Yu [VerfasserIn] |
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| Links: |
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| Themen: |
Anticoagulants |
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| Anmerkungen: |
Date Completed 01.02.2023 Date Revised 02.02.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.cca.2022.12.004 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM350151156 |
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| 520 | |a Copyright © 2022 Elsevier B.V. All rights reserved. | ||
| 520 | |a Antemortem specimens are sometimes the sole sources available for forensic investigation, and samples collected in nonideal ways are inevitably employed to achieve toxicological analysis. It is essential to assess the effects of blood collection tubes on the recoveries of emerging synthetic cathinones (SC) to estimate actual drug concentrations, and no such systematic investigations have been previously carried out. Seventy-one SC with various LogP values were employed to examine commonly used blood collection tubes, including plasma tubes, serum tubes and gel-containing tubes in recoveries which determined by a reliable LC-MS/MS method. Significantly poor recoveries for hydrophobic SC were obtained using serum separating tubes (SST). Notably, the suppressed recoveries in SST can be reversed by adding anticoagulants. Adding a procoagulant to a plasma separating tube (PST) considerably reduced recoveries, which indicated that clotting processes in the presence of polymeric gels contributed to poor recoveries of these hydrophobic drugs. In this study, we find that clotting formation in the presence of polymeric gels could significantly affect the determination of hydrophobic drugs. However, in real-world scenarios, nonideal collection methods are inevitably employed for antemortem specimens. Thus, it is important to rigorously interpret forensic toxicological results, especially for susceptible species | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Blood collection tubes | |
| 650 | 4 | |a Forensic toxicology | |
| 650 | 4 | |a LC-MS/MS | |
| 650 | 4 | |a Synthetic cathinones | |
| 650 | 7 | |a Synthetic Cathinone |2 NLM | |
| 650 | 7 | |a Anticoagulants |2 NLM | |
| 650 | 7 | |a Gels |2 NLM | |
| 700 | 1 | |a Chen, Guan-Yuan |e verfasserin |4 aut | |
| 700 | 1 | |a Ong, Hooi-Nee |e verfasserin |4 aut | |
| 700 | 1 | |a Lai, Mei-Ling |e verfasserin |4 aut | |
| 700 | 1 | |a Ho, Yi-Ju |e verfasserin |4 aut | |
| 700 | 1 | |a Kuo, Ching-Hua |e verfasserin |4 aut | |
| 700 | 1 | |a Weng, Te-I |e verfasserin |4 aut | |
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