Mesenchymal stem cells-derived exosomal miR-653-5p suppresses laryngeal papilloma progression by inhibiting BZW2
Copyright © 2022 HCFMUSP. Published by Elsevier España, S.L.U. All rights reserved..
OBJECTIVES: Although miR-653-5p has been validated to participate in the progression of multiple types of cancer, the functional role of exosomal miR-653-5p derived from Mesenchymal Stem Cells (MSCs) in Laryngeal Papilloma (LP) has still remained elusive. Hence, this study aimed to investigate the role of MSCs-derived exosomal miR-653-5p in LP.
METHODS: LP tissues (n = 15) and adjacent normal tissues (n = 10) were collected to examine the expression level of miR-653-5p. The expression level of miR-653-5p in LP cells and normal cells was also detected. Then, miR-653-5p was overexpressed or silenced to explore its effects on the proliferation, migration, invasion, and apoptosis of LP cells. Thereafter, the effects of exosomal miR-653-5p derived from MSCs on LP cell progression and the potential regulatory mechanism of miR-653-5p were assessed.
RESULTS: It was revealed that the expression level of miR-653-5p was downregulated in LP tissues and cells. In addition, miR-653-5p suppressed the proliferation, migration, invasion, and apoptosis of LP cells. Exosomes derived from MSCs played a suppressive role in LP development and mediated the transmission of miR-653-5p to LP cells. Further exploration identified Basic leucine Zipper and W2 domains 2 (BZW2) as the target of miR-653-5p. More importantly, the rescue experiments revealed that MSCs-secreted exosomal miR-653-5p efficiently inhibited the aggressive phenotypes of LP cells, which could be significantly reversed by BZW2 overexpression in LP cells.
CONCLUSION: MSCs-derived exosomal miR-653-5p exerted inhibitory effects on LP progression through targeting BZW2, which provided a novel idea for the therapy of LP.
CLINICAL TRIAL REGISTRATION NUMBER: chictr-ior-17011021.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:78 |
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| Enthalten in: |
Clinics (Sao Paulo, Brazil) - 78(2023) vom: 01., Seite 100129 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hu, Binya [VerfasserIn] |
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| Links: |
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| Themen: |
BZW2 protein, human |
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| Anmerkungen: |
Date Completed 21.12.2022 Date Revised 22.12.2022 published: Electronic-eCollection Citation Status MEDLINE |
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| doi: |
10.1016/j.clinsp.2022.100129 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM349857989 |
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| 245 | 1 | 0 | |a Mesenchymal stem cells-derived exosomal miR-653-5p suppresses laryngeal papilloma progression by inhibiting BZW2 |
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| 500 | |a Date Revised 22.12.2022 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2022 HCFMUSP. Published by Elsevier España, S.L.U. All rights reserved. | ||
| 520 | |a OBJECTIVES: Although miR-653-5p has been validated to participate in the progression of multiple types of cancer, the functional role of exosomal miR-653-5p derived from Mesenchymal Stem Cells (MSCs) in Laryngeal Papilloma (LP) has still remained elusive. Hence, this study aimed to investigate the role of MSCs-derived exosomal miR-653-5p in LP | ||
| 520 | |a METHODS: LP tissues (n = 15) and adjacent normal tissues (n = 10) were collected to examine the expression level of miR-653-5p. The expression level of miR-653-5p in LP cells and normal cells was also detected. Then, miR-653-5p was overexpressed or silenced to explore its effects on the proliferation, migration, invasion, and apoptosis of LP cells. Thereafter, the effects of exosomal miR-653-5p derived from MSCs on LP cell progression and the potential regulatory mechanism of miR-653-5p were assessed | ||
| 520 | |a RESULTS: It was revealed that the expression level of miR-653-5p was downregulated in LP tissues and cells. In addition, miR-653-5p suppressed the proliferation, migration, invasion, and apoptosis of LP cells. Exosomes derived from MSCs played a suppressive role in LP development and mediated the transmission of miR-653-5p to LP cells. Further exploration identified Basic leucine Zipper and W2 domains 2 (BZW2) as the target of miR-653-5p. More importantly, the rescue experiments revealed that MSCs-secreted exosomal miR-653-5p efficiently inhibited the aggressive phenotypes of LP cells, which could be significantly reversed by BZW2 overexpression in LP cells | ||
| 520 | |a CONCLUSION: MSCs-derived exosomal miR-653-5p exerted inhibitory effects on LP progression through targeting BZW2, which provided a novel idea for the therapy of LP | ||
| 520 | |a CLINICAL TRIAL REGISTRATION NUMBER: chictr-ior-17011021 | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Exosomes | |
| 650 | 4 | |a Laryngeal Diseases | |
| 650 | 4 | |a Mesenchymal Stem Cells | |
| 650 | 4 | |a MicroRNAs | |
| 650 | 4 | |a Papillomatosis | |
| 650 | 7 | |a MicroRNAs |2 NLM | |
| 650 | 7 | |a BZW2 protein, human |2 NLM | |
| 650 | 7 | |a DNA-Binding Proteins |2 NLM | |
| 700 | 1 | |a Huang, Min |e verfasserin |4 aut | |
| 700 | 1 | |a Tao, Lihua |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Yun |e verfasserin |4 aut | |
| 700 | 1 | |a Kuang, Yuting |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Guangliang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Sijun |e verfasserin |4 aut | |
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