Exosomal MicroRNAs : Comprehensive Methods from Exosome Isolation to miRNA Extraction and Purity Analysis
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature..
Exosomes are extracellular vesicles secreted by cells with a key role in a wide range of biological processes including cancer. These vesicles are involved in intercellular communication and deliver diverse cargo molecules, including miRNAs (exo-miRNAs), to recipient cells affecting their physiology. Exo-miRNAs have a role in promoting tumor, progression, metastatization, and remodeling of tumor microenvironment, therefore making them interesting biomarkers to study.Here we provide a detailed technical protocol for exosome isolation (which can be applied to cell culture as well as physiological fluids), validation of their vesicular identity, miRNA extraction, and quantitative and qualitative analysis to evaluate the sample purity and concentration.
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2595 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2595(2023) vom: 28., Seite 75-92 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
D'Agostino, Erika [VerfasserIn] |
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| Links: |
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| Themen: |
Differential ultracentrifugation |
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Date Completed 30.11.2022 Date Revised 28.01.2023 published: Print ErratumIn: Methods Mol Biol. 2023;2595:C1. - PMID 36708460 Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-2823-2_5 |
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| 520 | |a Exosomes are extracellular vesicles secreted by cells with a key role in a wide range of biological processes including cancer. These vesicles are involved in intercellular communication and deliver diverse cargo molecules, including miRNAs (exo-miRNAs), to recipient cells affecting their physiology. Exo-miRNAs have a role in promoting tumor, progression, metastatization, and remodeling of tumor microenvironment, therefore making them interesting biomarkers to study.Here we provide a detailed technical protocol for exosome isolation (which can be applied to cell culture as well as physiological fluids), validation of their vesicular identity, miRNA extraction, and quantitative and qualitative analysis to evaluate the sample purity and concentration | ||
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| 650 | 4 | |a Exosome RNA extraction | |
| 650 | 4 | |a Exosome RNA quality control | |
| 650 | 4 | |a Exosome characterization | |
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