Co-display of diverse spike proteins on nanoparticles broadens sarbecovirus neutralizing antibody responses
© 2022 The Author(s)..
The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants poses continuous challenges in combating the virus. Here, we describe vaccination strategies to broaden SARS-CoV-2 and sarbecovirus immunity by combining spike proteins based on different viruses or viral strains displayed on two-component protein nanoparticles. First, we combined spike proteins based on ancestral and Beta SARS-CoV-2 strains to broaden SARS-CoV-2 immune responses. Inclusion of Beta spike improved neutralizing antibody responses against SARS-CoV-2 Beta, Gamma, and Omicron BA.1 and BA.4/5. A third vaccination with ancestral SARS-CoV-2 spike also improved cross-neutralizing antibody responses against SARS-CoV-2 variants, in particular against the Omicron sublineages. Second, we combined SARS-CoV and SARS-CoV-2 spike proteins to broaden sarbecovirus immune responses. Adding SARS-CoV spike to a SARS-CoV-2 spike vaccine improved neutralizing responses against SARS-CoV and SARS-like bat sarbecoviruses SHC014 and WIV1. These results should inform the development of broadly active SARS-CoV-2 and pan-sarbecovirus vaccines and highlight the versatility of two-component nanoparticles for displaying diverse antigens.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:25 |
|---|---|
| Enthalten in: |
iScience - 25(2022), 12 vom: 22. Dez., Seite 105649 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Brinkkemper, Mitch [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Immune response |
|---|
| Anmerkungen: |
Date Revised 06.11.2023 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.1016/j.isci.2022.105649 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM349522774 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM349522774 | ||
| 003 | DE-627 | ||
| 005 | 20231226042833.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.isci.2022.105649 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1165.xml |
| 035 | |a (DE-627)NLM349522774 | ||
| 035 | |a (NLM)36439375 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Brinkkemper, Mitch |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Co-display of diverse spike proteins on nanoparticles broadens sarbecovirus neutralizing antibody responses |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 06.11.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © 2022 The Author(s). | ||
| 520 | |a The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants poses continuous challenges in combating the virus. Here, we describe vaccination strategies to broaden SARS-CoV-2 and sarbecovirus immunity by combining spike proteins based on different viruses or viral strains displayed on two-component protein nanoparticles. First, we combined spike proteins based on ancestral and Beta SARS-CoV-2 strains to broaden SARS-CoV-2 immune responses. Inclusion of Beta spike improved neutralizing antibody responses against SARS-CoV-2 Beta, Gamma, and Omicron BA.1 and BA.4/5. A third vaccination with ancestral SARS-CoV-2 spike also improved cross-neutralizing antibody responses against SARS-CoV-2 variants, in particular against the Omicron sublineages. Second, we combined SARS-CoV and SARS-CoV-2 spike proteins to broaden sarbecovirus immune responses. Adding SARS-CoV spike to a SARS-CoV-2 spike vaccine improved neutralizing responses against SARS-CoV and SARS-like bat sarbecoviruses SHC014 and WIV1. These results should inform the development of broadly active SARS-CoV-2 and pan-sarbecovirus vaccines and highlight the versatility of two-component nanoparticles for displaying diverse antigens | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Immune response | |
| 650 | 4 | |a Immunology | |
| 650 | 4 | |a Microbiology | |
| 650 | 4 | |a Virology | |
| 700 | 1 | |a Veth, Tim S |e verfasserin |4 aut | |
| 700 | 1 | |a Brouwer, Philip J M |e verfasserin |4 aut | |
| 700 | 1 | |a Turner, Hannah |e verfasserin |4 aut | |
| 700 | 1 | |a Poniman, Meliawati |e verfasserin |4 aut | |
| 700 | 1 | |a Burger, Judith A |e verfasserin |4 aut | |
| 700 | 1 | |a Bouhuijs, Joey H |e verfasserin |4 aut | |
| 700 | 1 | |a Olijhoek, Wouter |e verfasserin |4 aut | |
| 700 | 1 | |a Bontjer, Ilja |e verfasserin |4 aut | |
| 700 | 1 | |a Snitselaar, Jonne L |e verfasserin |4 aut | |
| 700 | 1 | |a Caniels, Tom G |e verfasserin |4 aut | |
| 700 | 1 | |a van der Linden, Cynthia A |e verfasserin |4 aut | |
| 700 | 1 | |a Ravichandran, Rashmi |e verfasserin |4 aut | |
| 700 | 1 | |a Villaudy, Julien |e verfasserin |4 aut | |
| 700 | 1 | |a van der Velden, Yme U |e verfasserin |4 aut | |
| 700 | 1 | |a Sliepen, Kwinten |e verfasserin |4 aut | |
| 700 | 1 | |a van Gils, Marit J |e verfasserin |4 aut | |
| 700 | 1 | |a Ward, Andrew B |e verfasserin |4 aut | |
| 700 | 1 | |a King, Neil P |e verfasserin |4 aut | |
| 700 | 1 | |a Heck, Albert J R |e verfasserin |4 aut | |
| 700 | 1 | |a Sanders, Rogier W |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t iScience |d 2018 |g 25(2022), 12 vom: 22. Dez., Seite 105649 |w (DE-627)NLM285332627 |x 2589-0042 |7 nnns |
| 773 | 1 | 8 | |g volume:25 |g year:2022 |g number:12 |g day:22 |g month:12 |g pages:105649 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.isci.2022.105649 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 25 |j 2022 |e 12 |b 22 |c 12 |h 105649 | ||