When yeast cells change their mind : cell cycle "Start" is reversible under starvation
© 2022 The Authors. Published under the terms of the CC BY NC ND 4.0 license..
Eukaryotic cells decide in late G1 phase of the cell cycle whether to commit to another round of division. This point of cell cycle commitment is termed "Restriction Point" in mammals and "Start" in the budding yeast Saccharomyces cerevisiae. At Start, yeast cells integrate multiple signals such as pheromones and nutrients, and will not pass Start if nutrients are lacking. However, how cells respond to nutrient depletion after the Start decision remains poorly understood. Here, we analyze how post-Start cells respond to nutrient depletion, by monitoring Whi5, the cell cycle inhibitor whose export from the nucleus determines Start. Surprisingly, we find that cells that have passed Start can re-import Whi5 into the nucleus. In these cells, the positive feedback loop activating G1/S transcription is interrupted, and the Whi5 repressor re-binds DNA. Cells which re-import Whi5 become again sensitive to mating pheromone, like pre-Start cells, and CDK activation can occur a second time upon replenishment of nutrients. These results demonstrate that upon starvation, the commitment decision at Start can be reversed. We therefore propose that cell cycle commitment in yeast is a multi-step process, similar to what has been suggested for mammalian cells.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:42 |
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| Enthalten in: |
The EMBO journal - 42(2023), 2 vom: 16. Jan., Seite e110321 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Irvali, Deniz [VerfasserIn] |
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| Links: |
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| Themen: |
Cell division cycle |
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| Anmerkungen: |
Date Completed 17.01.2023 Date Revised 14.03.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.15252/embj.2021110321 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM349336334 |
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| 245 | 1 | 0 | |a When yeast cells change their mind |b cell cycle "Start" is reversible under starvation |
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| 500 | |a Date Revised 14.03.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2022 The Authors. Published under the terms of the CC BY NC ND 4.0 license. | ||
| 520 | |a Eukaryotic cells decide in late G1 phase of the cell cycle whether to commit to another round of division. This point of cell cycle commitment is termed "Restriction Point" in mammals and "Start" in the budding yeast Saccharomyces cerevisiae. At Start, yeast cells integrate multiple signals such as pheromones and nutrients, and will not pass Start if nutrients are lacking. However, how cells respond to nutrient depletion after the Start decision remains poorly understood. Here, we analyze how post-Start cells respond to nutrient depletion, by monitoring Whi5, the cell cycle inhibitor whose export from the nucleus determines Start. Surprisingly, we find that cells that have passed Start can re-import Whi5 into the nucleus. In these cells, the positive feedback loop activating G1/S transcription is interrupted, and the Whi5 repressor re-binds DNA. Cells which re-import Whi5 become again sensitive to mating pheromone, like pre-Start cells, and CDK activation can occur a second time upon replenishment of nutrients. These results demonstrate that upon starvation, the commitment decision at Start can be reversed. We therefore propose that cell cycle commitment in yeast is a multi-step process, similar to what has been suggested for mammalian cells | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Saccharomyces cerevisiae | |
| 650 | 4 | |a cell division cycle | |
| 650 | 4 | |a metabolism | |
| 650 | 4 | |a nutrient signaling | |
| 650 | 4 | |a quiescence | |
| 650 | 7 | |a Saccharomyces cerevisiae Proteins |2 NLM | |
| 700 | 1 | |a Schlottmann, Fabian P |e verfasserin |4 aut | |
| 700 | 1 | |a Muralidhara, Prathibha |e verfasserin |4 aut | |
| 700 | 1 | |a Nadelson, Iliya |e verfasserin |4 aut | |
| 700 | 1 | |a Kleemann, Katja |e verfasserin |4 aut | |
| 700 | 1 | |a Wood, N Ezgi |e verfasserin |4 aut | |
| 700 | 1 | |a Doncic, Andreas |e verfasserin |4 aut | |
| 700 | 1 | |a Ewald, Jennifer C |e verfasserin |4 aut | |
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