ZEB1-activated Notch1 promotes circulating tumor cell migration and invasion in lung squamous cell carcinoma
© 2022. The Author(s), under exclusive licence to Federación de Sociedades Españolas de Oncología (FESEO)..
BACKGROUND: Lung squamous cell carcinoma (LUSC) is recognized as the major subtypes of non-small cell lung cancer (NSCLC). Circulating tumor cells (CTCs) are critical players in tumor metastasis. A molecular profiling of CTCs has previously identified notch receptor 1 (Notch1) as an important mediator in NSCLC. Therefore, we investigate Notch1 roles in LUSC and its related mechanisms.
METHODS: The serum levels of Notch1 were measured by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The CTCs isolated from blood samples were characterized via an immunofluorescence method. Cell motion was determined using Transwell chambers. The regulatory relationship between Notch1 and zinc finger E-box-binding homeobox 1 (ZEB1) was verified by chromatin immunoprecipitation (ChIP) and luciferase reporter assays. The protein levels were detected by western blotting.
RESULTS: Higher Notch1 expression in patients with LUSC than that in normal controls was observed. Notch1 knockdown inhibited cell motion and epithelial-mesenchymal transition (EMT). ZEB1 transcriptionally activated Notch1. ZEB1 upregulation exacerbated the malignant phenotypes of CTCs.
CONCLUSION: ZEB1-activated Notch1 promotes malignant phenotypes of CTCs in LUSC and indicates poor prognosis.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2023 |
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Erschienen: |
2023 |
Enthalten in: |
Zur Gesamtaufnahme - volume:25 |
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Enthalten in: |
Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico - 25(2023), 3 vom: 22. März, Seite 817-829 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Gao, Yong [VerfasserIn] |
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Links: |
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Anmerkungen: |
Date Completed 23.02.2023 Date Revised 23.02.2023 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1007/s12094-022-02993-7 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM349317364 |
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500 | |a Citation Status MEDLINE | ||
520 | |a © 2022. The Author(s), under exclusive licence to Federación de Sociedades Españolas de Oncología (FESEO). | ||
520 | |a BACKGROUND: Lung squamous cell carcinoma (LUSC) is recognized as the major subtypes of non-small cell lung cancer (NSCLC). Circulating tumor cells (CTCs) are critical players in tumor metastasis. A molecular profiling of CTCs has previously identified notch receptor 1 (Notch1) as an important mediator in NSCLC. Therefore, we investigate Notch1 roles in LUSC and its related mechanisms | ||
520 | |a METHODS: The serum levels of Notch1 were measured by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The CTCs isolated from blood samples were characterized via an immunofluorescence method. Cell motion was determined using Transwell chambers. The regulatory relationship between Notch1 and zinc finger E-box-binding homeobox 1 (ZEB1) was verified by chromatin immunoprecipitation (ChIP) and luciferase reporter assays. The protein levels were detected by western blotting | ||
520 | |a RESULTS: Higher Notch1 expression in patients with LUSC than that in normal controls was observed. Notch1 knockdown inhibited cell motion and epithelial-mesenchymal transition (EMT). ZEB1 transcriptionally activated Notch1. ZEB1 upregulation exacerbated the malignant phenotypes of CTCs | ||
520 | |a CONCLUSION: ZEB1-activated Notch1 promotes malignant phenotypes of CTCs in LUSC and indicates poor prognosis | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Circulating tumor cell | |
650 | 4 | |a Lung squamous cell carcinoma | |
650 | 4 | |a Notch receptor 1 (Notch1) | |
650 | 4 | |a Zinc finger E-box-binding homeobox 1 (ZEB1) | |
650 | 7 | |a MicroRNAs |2 NLM | |
650 | 7 | |a ZEB1 protein, human |2 NLM | |
650 | 7 | |a Zinc Finger E-box-Binding Homeobox 1 |2 NLM | |
650 | 7 | |a NOTCH1 protein, human |2 NLM | |
650 | 7 | |a Receptor, Notch1 |2 NLM | |
700 | 1 | |a Cheng, Xinyuan |e verfasserin |4 aut | |
700 | 1 | |a Han, Mingfeng |e verfasserin |4 aut | |
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