GPI-anchored ligand-BioID2-tagging system identifies Galectin-1 mediating Zika virus entry
© 2022 The Author(s)..
Identification of host factors facilitating pathogen entry is critical for preventing infectious diseases. Here, we report a tagging system consisting of a viral receptor-binding protein (RBP) linked to BioID2, which is expressed on the cell surface via a GPI anchor. Using VSV or Zika virus (ZIKV) RBP, the system (BioID2- RBP(V)-GPI; BioID2-RBP(Z)-GPI) faithfully identifies LDLR and AXL, the receptors of VSV and ZIKV, respectively. Being GPI-anchored is essential for the probe to function properly. Furthermore, BioID2-RBP(Z)-GPI expressed in human neuronal progenitor cells identifies galectin-1 on cell surface pivotal for ZIKV entry. This conclusion is further supported by antibody blocking and galectin-1 silencing in A549 and mouse neural cells. Importantly, Lgals1 -/- mice are significantly more resistant to ZIKV infection than Lgals1 +/+ littermates are, having significantly lower virus titers and fewer pathologies in various organs. This tagging system may have broad applications for identifying protein-protein interactions on the cell surface.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:25 |
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| Enthalten in: |
iScience - 25(2022), 12 vom: 22. Dez., Seite 105481 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Gao, Shan-Shan [VerfasserIn] |
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| Links: |
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| Themen: |
Biological sciences |
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| Anmerkungen: |
Date Revised 22.11.2022 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1016/j.isci.2022.105481 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM349181179 |
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| 520 | |a © 2022 The Author(s). | ||
| 520 | |a Identification of host factors facilitating pathogen entry is critical for preventing infectious diseases. Here, we report a tagging system consisting of a viral receptor-binding protein (RBP) linked to BioID2, which is expressed on the cell surface via a GPI anchor. Using VSV or Zika virus (ZIKV) RBP, the system (BioID2- RBP(V)-GPI; BioID2-RBP(Z)-GPI) faithfully identifies LDLR and AXL, the receptors of VSV and ZIKV, respectively. Being GPI-anchored is essential for the probe to function properly. Furthermore, BioID2-RBP(Z)-GPI expressed in human neuronal progenitor cells identifies galectin-1 on cell surface pivotal for ZIKV entry. This conclusion is further supported by antibody blocking and galectin-1 silencing in A549 and mouse neural cells. Importantly, Lgals1 -/- mice are significantly more resistant to ZIKV infection than Lgals1 +/+ littermates are, having significantly lower virus titers and fewer pathologies in various organs. This tagging system may have broad applications for identifying protein-protein interactions on the cell surface | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Biological sciences | |
| 650 | 4 | |a Microbiology | |
| 650 | 4 | |a Molecular biology | |
| 650 | 4 | |a Virology | |
| 700 | 1 | |a Shi, Run |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Tang, Yanhong |e verfasserin |4 aut | |
| 700 | 1 | |a Zheng, Zhenhua |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jing-Feng |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Huan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Jie |e verfasserin |4 aut | |
| 700 | 1 | |a Leng, Qibin |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Jiang |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Xinwen |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Jincun |e verfasserin |4 aut | |
| 700 | 1 | |a Sy, Man-Sun |e verfasserin |4 aut | |
| 700 | 1 | |a Feng, Liqiang |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Chaoyang |e verfasserin |4 aut | |
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