Laser photobiomodulation does not alter clinical and histological characteristics of 4-NQO-induced oral carcinomas and leukoplakia in mice
Copyright © 2022 Elsevier B.V. All rights reserved..
OBJECTIVE: This study evaluated the effect of laser photobiomodulation (PBM) on oral leukoplakia and squamous cell carcinomas (OSCC) in a model of oral carcinogenesis.
MATERIALS AND METHODS: Forty-one C57Bl/6 female mice were distributed in control group, 4-NQO group, Laser group 1.5 J and Laser group 9 J. Oral cancer was induced on the tongue by nitroquinoline oxide (4-NQO), diluted in the water for 16 weeks. In the 18th and 19th weeks, PBM with a diode laser, 0.028 cm2 spot size, continuous emission mode, 660 nm wavelength was applied on the tongue of animals for seven sessions. Laser group 1.5 J received 30 mW power and 1.5 J energy. In the Laser group 9 J, 100 mW power, and 9 J energy were applied. In the 20th week the animals were euthanized.
RESULTS: All animals exposed to carcinogen developed clinical and histological alterations such as leukoplakia and OSCC on the tongue. There was no significant difference among Laser groups 1.5 and 9 J and 4-NQO group (not irradiated) regarding the area of leukoplakia and carcinomas (P > 0.05) or thickness of epithelial tissue and keratin (P > 0.05). There were also no association between PBM and histologic classification of the lesions (P = 0.87), frequency of OSCC (P = 0.57), grade of tumor differentiation (P = 0.88) or depth of invasion (P = 0.45).
CONCLUSION: Laser PBM, in both parameters used, does not influence on clinical and histological characteristics of oral leukoplakia and OSCC.
CLINICAL RELEVANCE: Results suggest that PBM may be a safe treatment for adverse effects of antineoplastic therapies in patients with leukoplakia and OSCC.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:237 |
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| Enthalten in: |
Journal of photochemistry and photobiology. B, Biology - 237(2022) vom: 15. Dez., Seite 112597 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Neculqueo, Gabriela Weirich [VerfasserIn] |
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| Links: |
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| Themen: |
4-NQO |
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| Anmerkungen: |
Date Completed 02.12.2022 Date Revised 02.12.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jphotobiol.2022.112597 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 100 | 1 | |a Neculqueo, Gabriela Weirich |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Laser photobiomodulation does not alter clinical and histological characteristics of 4-NQO-induced oral carcinomas and leukoplakia in mice |
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| 500 | |a Date Revised 02.12.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2022 Elsevier B.V. All rights reserved. | ||
| 520 | |a OBJECTIVE: This study evaluated the effect of laser photobiomodulation (PBM) on oral leukoplakia and squamous cell carcinomas (OSCC) in a model of oral carcinogenesis | ||
| 520 | |a MATERIALS AND METHODS: Forty-one C57Bl/6 female mice were distributed in control group, 4-NQO group, Laser group 1.5 J and Laser group 9 J. Oral cancer was induced on the tongue by nitroquinoline oxide (4-NQO), diluted in the water for 16 weeks. In the 18th and 19th weeks, PBM with a diode laser, 0.028 cm2 spot size, continuous emission mode, 660 nm wavelength was applied on the tongue of animals for seven sessions. Laser group 1.5 J received 30 mW power and 1.5 J energy. In the Laser group 9 J, 100 mW power, and 9 J energy were applied. In the 20th week the animals were euthanized | ||
| 520 | |a RESULTS: All animals exposed to carcinogen developed clinical and histological alterations such as leukoplakia and OSCC on the tongue. There was no significant difference among Laser groups 1.5 and 9 J and 4-NQO group (not irradiated) regarding the area of leukoplakia and carcinomas (P > 0.05) or thickness of epithelial tissue and keratin (P > 0.05). There were also no association between PBM and histologic classification of the lesions (P = 0.87), frequency of OSCC (P = 0.57), grade of tumor differentiation (P = 0.88) or depth of invasion (P = 0.45) | ||
| 520 | |a CONCLUSION: Laser PBM, in both parameters used, does not influence on clinical and histological characteristics of oral leukoplakia and OSCC | ||
| 520 | |a CLINICAL RELEVANCE: Results suggest that PBM may be a safe treatment for adverse effects of antineoplastic therapies in patients with leukoplakia and OSCC | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a 4-NQO | |
| 650 | 4 | |a Mice | |
| 650 | 4 | |a Oral carcinogenesis | |
| 650 | 4 | |a Photobiomodulation | |
| 650 | 7 | |a 4-Nitroquinoline-1-oxide |2 NLM | |
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| 650 | 7 | |a Carcinogens |2 NLM | |
| 700 | 1 | |a Estrázulas, Marina |e verfasserin |4 aut | |
| 700 | 1 | |a Cherubini, Karen |e verfasserin |4 aut | |
| 700 | 1 | |a Koth, Valesca Sander |e verfasserin |4 aut | |
| 700 | 1 | |a Salum, Fernanda Gonçalves |e verfasserin |4 aut | |
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