Dually innervated dendritic spines develop in the absence of excitatory activity and resist plasticity through tonic inhibitory crosstalk
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved..
Dendritic spines can be directly connected to both inhibitory and excitatory presynaptic terminals, resulting in nanometer-scale proximity of opposing synaptic functions. While dually innervated spines (DiSs) are observed throughout the central nervous system, their developmental timeline and functional properties remain uncharacterized. Here we used a combination of serial section electron microscopy, live imaging, and local synapse activity manipulations to investigate DiS development and function in rodent hippocampus. Dual innervation occurred early in development, even on spines where the excitatory input was locally silenced. Synaptic NMDA receptor currents were selectively reduced at DiSs through tonic GABAB receptor signaling. Accordingly, spine enlargement normally associated with long-term potentiation on singly innervated spines (SiSs) was blocked at DiSs. Silencing somatostatin interneurons or pharmacologically blocking GABABRs restored NMDA receptor function and structural plasticity to levels comparable to neighboring SiSs. Thus, hippocampal DiSs are stable structures where function and plasticity are potently regulated by nanometer-scale GABAergic signaling.
| Errataetall: | |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:111 |
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| Enthalten in: |
Neuron - 111(2023), 3 vom: 01. Feb., Seite 362-371.e6 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kleinjan, Mason S [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 06.02.2023 Date Revised 02.02.2024 published: Print-Electronic ErratumIn: Neuron. 2023 May 3;111(9):1517. - PMID 37141861 Citation Status MEDLINE |
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| doi: |
10.1016/j.neuron.2022.11.002 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM349090653 |
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| 100 | 1 | |a Kleinjan, Mason S |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Dually innervated dendritic spines develop in the absence of excitatory activity and resist plasticity through tonic inhibitory crosstalk |
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| 500 | |a Date Revised 02.02.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a ErratumIn: Neuron. 2023 May 3;111(9):1517. - PMID 37141861 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a Dendritic spines can be directly connected to both inhibitory and excitatory presynaptic terminals, resulting in nanometer-scale proximity of opposing synaptic functions. While dually innervated spines (DiSs) are observed throughout the central nervous system, their developmental timeline and functional properties remain uncharacterized. Here we used a combination of serial section electron microscopy, live imaging, and local synapse activity manipulations to investigate DiS development and function in rodent hippocampus. Dual innervation occurred early in development, even on spines where the excitatory input was locally silenced. Synaptic NMDA receptor currents were selectively reduced at DiSs through tonic GABAB receptor signaling. Accordingly, spine enlargement normally associated with long-term potentiation on singly innervated spines (SiSs) was blocked at DiSs. Silencing somatostatin interneurons or pharmacologically blocking GABABRs restored NMDA receptor function and structural plasticity to levels comparable to neighboring SiSs. Thus, hippocampal DiSs are stable structures where function and plasticity are potently regulated by nanometer-scale GABAergic signaling | ||
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| 700 | 1 | |a Kuwajima, Masaaki |e verfasserin |4 aut | |
| 700 | 1 | |a Hubbard, Dusten D |e verfasserin |4 aut | |
| 700 | 1 | |a Kareemo, Dean J |e verfasserin |4 aut | |
| 700 | 1 | |a Prikhodko, Olga |e verfasserin |4 aut | |
| 700 | 1 | |a Olah, Samantha L |e verfasserin |4 aut | |
| 700 | 1 | |a Gomez Wulschner, Luis E |e verfasserin |4 aut | |
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| 700 | 1 | |a Oh, Won Chan |e verfasserin |4 aut | |
| 700 | 1 | |a Kennedy, Matthew J |e verfasserin |4 aut | |
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