Differential activation of human neutrophils by SARS-CoV-2 variants of concern
Copyright © 2022 Lebourgeois, David, Chenane, Granger, Menidjel, Fidouh, Noël, Delelis, Richetta, Charpentier, Chollet-Martin, Descamps, Visseaux and de Chaisemartin..
The emerging SARS-CoV-2 virus has affected the entire world with over 600 million confirmed cases and 6.5 million deaths as of September 2022. Since the beginning of the pandemic, several variants of SARS-CoV-2 have emerged, with different infectivity and virulence. Several studies suggest an important role of neutrophils in SARS-Cov-2 infection severity, but data about direct activation of neutrophils by the virus is scarce. Here, we studied the in vitro activation of human neutrophils by SARS-CoV-2 variants of concern (VOCs). In our work, we show that upon stimulation with SARS-Cov-2 infectious particles, human healthy resting neutrophils upregulate activation markers, degranulate IL-8, produce Reactive Oxygen Species and release Neutrophil Extracellular Traps. Neutrophil activation was dependent on TLR7/8 and IRF3/STING. We then compared the activation potential of neutrophils by SARS-CoV-2 variants and showed a significantly increased activation by the Delta variant and a decreased activation by the Omicron variant as compared to the initial strain. In this study, we demonstrate that the SARS-Cov-2 virus can directly activate neutrophils in COVID-19 and that the different VOCs had differences in neutrophil activation intensity that mirror the differences of clinical severity. These data highlight the need to address neutrophil-virus interactions as a potential target for therapeutic intervention in SARS-CoV-2 infection.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Frontiers in immunology - 13(2022) vom: 18., Seite 1010140 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lebourgeois, Samuel [VerfasserIn] |
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| Links: |
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| Themen: |
Human neutrophils |
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| Anmerkungen: |
Date Completed 21.11.2022 Date Revised 22.11.2022 published: Electronic-eCollection Citation Status MEDLINE |
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| doi: |
10.3389/fimmu.2022.1010140 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM349030731 |
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| 520 | |a The emerging SARS-CoV-2 virus has affected the entire world with over 600 million confirmed cases and 6.5 million deaths as of September 2022. Since the beginning of the pandemic, several variants of SARS-CoV-2 have emerged, with different infectivity and virulence. Several studies suggest an important role of neutrophils in SARS-Cov-2 infection severity, but data about direct activation of neutrophils by the virus is scarce. Here, we studied the in vitro activation of human neutrophils by SARS-CoV-2 variants of concern (VOCs). In our work, we show that upon stimulation with SARS-Cov-2 infectious particles, human healthy resting neutrophils upregulate activation markers, degranulate IL-8, produce Reactive Oxygen Species and release Neutrophil Extracellular Traps. Neutrophil activation was dependent on TLR7/8 and IRF3/STING. We then compared the activation potential of neutrophils by SARS-CoV-2 variants and showed a significantly increased activation by the Delta variant and a decreased activation by the Omicron variant as compared to the initial strain. In this study, we demonstrate that the SARS-Cov-2 virus can directly activate neutrophils in COVID-19 and that the different VOCs had differences in neutrophil activation intensity that mirror the differences of clinical severity. These data highlight the need to address neutrophil-virus interactions as a potential target for therapeutic intervention in SARS-CoV-2 infection | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a IL-8 | |
| 650 | 4 | |a SARS-CoV-2 | |
| 650 | 4 | |a human neutrophils | |
| 650 | 4 | |a netosis | |
| 650 | 4 | |a variant of concerns | |
| 700 | 1 | |a David, Ambroise |e verfasserin |4 aut | |
| 700 | 1 | |a Chenane, Houssem Redha |e verfasserin |4 aut | |
| 700 | 1 | |a Granger, Vanessa |e verfasserin |4 aut | |
| 700 | 1 | |a Menidjel, Reyene |e verfasserin |4 aut | |
| 700 | 1 | |a Fidouh, Nadhira |e verfasserin |4 aut | |
| 700 | 1 | |a Noël, Benoît |e verfasserin |4 aut | |
| 700 | 1 | |a Delelis, Olivier |e verfasserin |4 aut | |
| 700 | 1 | |a Richetta, Clémence |e verfasserin |4 aut | |
| 700 | 1 | |a Charpentier, Charlotte |e verfasserin |4 aut | |
| 700 | 1 | |a Chollet-Martin, Sylvie |e verfasserin |4 aut | |
| 700 | 1 | |a Descamps, Diane |e verfasserin |4 aut | |
| 700 | 1 | |a Visseaux, Benoit |e verfasserin |4 aut | |
| 700 | 1 | |a de Chaisemartin, Luc |e verfasserin |4 aut | |
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