Comparative study of commercial media to improve GMP manufacturing of recombinant human interferon β-1a by CHO cells in perfusion bioreactor
© The Author(s), under exclusive licence to Springer Nature B.V. 2022, Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law..
Chinese hamster ovary cells are the main cellular factories for production of a wide range of recombinant proteins in biopharmaceutical industry. Recombinant human Interferon beta-1a (rh-IFN β-1a), as a cytokine is broadly used to treat multiple sclerosis. In this work, the cell line producing rh-IFN β-1a was studied to improve cell density along with the specific expression. For this reason different cell culture experiments were done using different commercial serum-free media to find the appropriate media providing higher cell density. It was shown DMEMF12, DMEM:ProCHO5, and CHO-S-SFM II led to higher cell density and shorter doubling time. Next, using these media, fed-batch, and perfusion culture with temperature shift were implemented to investigate the best condition for industrial-scale manufacturing of rh-IFN β-1a in terms of higher cell density and product expression yield. The results demonstrated that CHO-S-SFM II media and a thermally biphasic condition provide enhanced expression of rh-IFN β-1a in perfusion bioreactor.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:74 |
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| Enthalten in: |
Cytotechnology - 74(2022), 6 vom: 05. Dez., Seite 669-680 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Sedighikamal, Hossein [VerfasserIn] |
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| Links: |
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| Themen: |
Chinese hamster ovary cells |
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| Anmerkungen: |
Date Revised 02.12.2023 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1007/s10616-022-00554-y |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Chinese hamster ovary cells are the main cellular factories for production of a wide range of recombinant proteins in biopharmaceutical industry. Recombinant human Interferon beta-1a (rh-IFN β-1a), as a cytokine is broadly used to treat multiple sclerosis. In this work, the cell line producing rh-IFN β-1a was studied to improve cell density along with the specific expression. For this reason different cell culture experiments were done using different commercial serum-free media to find the appropriate media providing higher cell density. It was shown DMEMF12, DMEM:ProCHO5, and CHO-S-SFM II led to higher cell density and shorter doubling time. Next, using these media, fed-batch, and perfusion culture with temperature shift were implemented to investigate the best condition for industrial-scale manufacturing of rh-IFN β-1a in terms of higher cell density and product expression yield. The results demonstrated that CHO-S-SFM II media and a thermally biphasic condition provide enhanced expression of rh-IFN β-1a in perfusion bioreactor | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Chinese hamster ovary cells | |
| 650 | 4 | |a Fed-batch | |
| 650 | 4 | |a Perfusion | |
| 650 | 4 | |a Rh-IFN β-1a | |
| 650 | 4 | |a Specific productivity | |
| 700 | 1 | |a Karimi Mostofi, Reza |e verfasserin |4 aut | |
| 700 | 1 | |a Sattarzadeh, Alireza |e verfasserin |4 aut | |
| 700 | 1 | |a Shahbazi, Mansour |e verfasserin |4 aut | |
| 700 | 1 | |a Aghazadeh, Hossein |e verfasserin |4 aut | |
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