Cerebellar irradiation does not cause hyperactivity, fear, and anxiety-related disorders in the juvenile rat brain
© 2022. The Author(s) under exclusive licence to European Society of Radiology..
BACKGROUND: The cerebellum is involved in hyperactivity, fear, and anxiety disorders that could be induced by whole-brain irradiation (WBI). However, whether cerebellar irradiation alone (CIA) could induce these disorders is unknown. We investigated the effect of CIA in an animal model.
METHODS: Eleven-day-old rat pups underwent a single 3-Gy dose of either WBI (n = 28) or CIA (n = 20), while 34 rat pups were sham-irradiated (controls). Cell death was evaluated in the subgranular zone of the hippocampus by counting pyknotic cells after haematoxylin/eosin staining at 6 h after irradiation for 10, 8, and 9 pups, respectively. Behavioural changes were evaluated via open-field test at 6 weeks for 18, 12, and 25 pups, respectively. Unpaired two-tailed t-test and one-way and two-way repeated ANOVA were used.
RESULTS: Massive cell death in cerebellar external granular layer was detected at 6 h after CIA (1,419 ± 211 mm, mean ± S.E.M. versus controls (68 ± 12 mm) (p < 0.001)), while no significant difference between CIA (1,419 ± 211 mm) versus WBI (1,433 ± 107 mm) (p = 0.955) was found. At open-field behavioural test, running distance, activity, wall distance, middle zone visit times, and duration were higher for WBI versus controls (p < 0.010), but no difference between CIA and controls was found (p > 0.05).
CONCLUSIONS: Although the cerebellum is involved in hyperactivity, fear, and anxiety disorders, CIA did not induce these disorders, indicating that WBI-induced cerebellar injury does not directly cause these behavioural abnormalities after WBI. Thus, targeting the cerebellum alone may not be enough to rescue or reduce these behavioural abnormalities after WBI.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:6 |
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| Enthalten in: |
European radiology experimental - 6(2022), 1 vom: 15. Nov., Seite 57 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Yafeng [VerfasserIn] |
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| Links: |
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| Themen: |
Anxiety |
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| Anmerkungen: |
Date Completed 16.11.2022 Date Revised 29.11.2022 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1186/s41747-022-00307-8 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 0 | |a Cerebellar irradiation does not cause hyperactivity, fear, and anxiety-related disorders in the juvenile rat brain |
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| 500 | |a Date Revised 29.11.2022 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2022. The Author(s) under exclusive licence to European Society of Radiology. | ||
| 520 | |a BACKGROUND: The cerebellum is involved in hyperactivity, fear, and anxiety disorders that could be induced by whole-brain irradiation (WBI). However, whether cerebellar irradiation alone (CIA) could induce these disorders is unknown. We investigated the effect of CIA in an animal model | ||
| 520 | |a METHODS: Eleven-day-old rat pups underwent a single 3-Gy dose of either WBI (n = 28) or CIA (n = 20), while 34 rat pups were sham-irradiated (controls). Cell death was evaluated in the subgranular zone of the hippocampus by counting pyknotic cells after haematoxylin/eosin staining at 6 h after irradiation for 10, 8, and 9 pups, respectively. Behavioural changes were evaluated via open-field test at 6 weeks for 18, 12, and 25 pups, respectively. Unpaired two-tailed t-test and one-way and two-way repeated ANOVA were used | ||
| 520 | |a RESULTS: Massive cell death in cerebellar external granular layer was detected at 6 h after CIA (1,419 ± 211 mm, mean ± S.E.M. versus controls (68 ± 12 mm) (p < 0.001)), while no significant difference between CIA (1,419 ± 211 mm) versus WBI (1,433 ± 107 mm) (p = 0.955) was found. At open-field behavioural test, running distance, activity, wall distance, middle zone visit times, and duration were higher for WBI versus controls (p < 0.010), but no difference between CIA and controls was found (p > 0.05) | ||
| 520 | |a CONCLUSIONS: Although the cerebellum is involved in hyperactivity, fear, and anxiety disorders, CIA did not induce these disorders, indicating that WBI-induced cerebellar injury does not directly cause these behavioural abnormalities after WBI. Thus, targeting the cerebellum alone may not be enough to rescue or reduce these behavioural abnormalities after WBI | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Anxiety | |
| 650 | 4 | |a Cerebellum | |
| 650 | 4 | |a Hyperactivity | |
| 650 | 4 | |a Models (animal) | |
| 650 | 4 | |a Radiotherapy | |
| 700 | 1 | |a Xie, Cuicui |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Yiran |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yaodong |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Changlian |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Kai |e verfasserin |4 aut | |
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