Disentangling the coupling relationships between functional denitrifiers and nitrogen transformation during cattle-manure and biochar composting : A novel perspective
Copyright © 2022 Elsevier Ltd. All rights reserved..
This study explored the coupling relationships between denitrifiers and N-transformation using multi-level (DNA, RNA and enzyme) and multi-aspect (abundance, diversity, structure, key community, network pattern, and functional module) analyses during cattle-manure (CM) and biochar (CMB) composting. Amino sugar-N (ASN, 0.914) and hydrolysable unknown-N (-0.724) were main organic-N components mediating NH4+-N in CM and CMB, respectively. Biochar lowered nirK, nirS, and nosZ genes copies, up-regulated nir gene transcripts, and inhibited nitrite reductase (NIR) activity. For nirK-denitrifiers, Luteimonas was predominant taxa influencing NO2--N and amino acid-N (AAN). Unclassified_k_norank_d_Bacteria and unclassified_p_Proteobacteria regulated NO3--N and ASN, respectively. These three genera played crucial roles in mediating NIR activity and nosZ/nirK. For nirS-denitrifiers, Paracoccus and Pseudomonas mediated NH4+-N and AAN, respectively, and they were vital genera regulating NO3--N, ASN and NIR activity. Furthermore, nirK-denitrifiers was major contributor to denitrification. Overall, functional denitrifiers might simultaneously participate in multiple N-transformation processes.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:367 |
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| Enthalten in: |
Bioresource technology - 367(2023) vom: 01. Jan., Seite 128235 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Deng, Liting [VerfasserIn] |
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| Links: |
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| Themen: |
Biochar |
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| Anmerkungen: |
Date Completed 23.11.2022 Date Revised 23.11.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.biortech.2022.128235 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a This study explored the coupling relationships between denitrifiers and N-transformation using multi-level (DNA, RNA and enzyme) and multi-aspect (abundance, diversity, structure, key community, network pattern, and functional module) analyses during cattle-manure (CM) and biochar (CMB) composting. Amino sugar-N (ASN, 0.914) and hydrolysable unknown-N (-0.724) were main organic-N components mediating NH4+-N in CM and CMB, respectively. Biochar lowered nirK, nirS, and nosZ genes copies, up-regulated nir gene transcripts, and inhibited nitrite reductase (NIR) activity. For nirK-denitrifiers, Luteimonas was predominant taxa influencing NO2--N and amino acid-N (AAN). Unclassified_k_norank_d_Bacteria and unclassified_p_Proteobacteria regulated NO3--N and ASN, respectively. These three genera played crucial roles in mediating NIR activity and nosZ/nirK. For nirS-denitrifiers, Paracoccus and Pseudomonas mediated NH4+-N and AAN, respectively, and they were vital genera regulating NO3--N, ASN and NIR activity. Furthermore, nirK-denitrifiers was major contributor to denitrification. Overall, functional denitrifiers might simultaneously participate in multiple N-transformation processes | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Biochar | |
| 650 | 4 | |a Denitrifying bacteria | |
| 650 | 4 | |a Multi-aspect analysis | |
| 650 | 4 | |a N-transformation | |
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| 700 | 1 | |a Liu, Wanying |e verfasserin |4 aut | |
| 700 | 1 | |a Chang, Nuo |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Lei |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Jizhou |e verfasserin |4 aut | |
| 700 | 1 | |a Bello, Ayodeji |e verfasserin |4 aut | |
| 700 | 1 | |a Uzoamaka Egbeagu, Ugochi |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Shuai |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Xiuhong |e verfasserin |4 aut | |
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