Human pre-60S assembly factors link rRNA transcription to pre-rRNA processing
Published by Cold Spring Harbor Laboratory Press for the RNA Society..
In eukaryotes, the nucleolus is the site of ribosome biosynthesis, an essential process in all cells. While human ribosome assembly is largely evolutionarily conserved, many of the regulatory details underlying its control and function have not yet been well-defined. The nucleolar protein RSL24D1 was originally identified as a factor important for 60S ribosomal subunit biogenesis. In addition, the PeBoW (BOP1-PES1-WDR12) complex has been well-defined as required for pre-28S rRNA processing and cell proliferation. In this study, we show that RSL24D1 depletion impairs both pre-ribosomal RNA (pre-rRNA) transcription and mature 28S rRNA production, leading to decreased protein synthesis and p53 stabilization in human cells. Surprisingly, each of the PeBoW complex members is also required for pre-rRNA transcription. We demonstrate that RSL24D1 and WDR12 co-immunoprecipitate with the RNA polymerase I subunit, RPA194, and regulate its steady state levels. These results uncover the dual role of RSL24D1 and the PeBoW complex in multiple steps of ribosome biogenesis, and provide evidence implicating large ribosomal subunit biogenesis factors in pre-rRNA transcription control.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2022 |
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| Enthalten in: |
RNA (New York, N.Y.) - (2022) vom: 02. Nov. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
McCool, Mason A [VerfasserIn] |
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| Links: |
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| Themen: |
60S large ribosomal subunit |
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| Anmerkungen: |
Date Revised 16.02.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1261/rna.079149.122 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM348374291 |
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| 520 | |a Published by Cold Spring Harbor Laboratory Press for the RNA Society. | ||
| 520 | |a In eukaryotes, the nucleolus is the site of ribosome biosynthesis, an essential process in all cells. While human ribosome assembly is largely evolutionarily conserved, many of the regulatory details underlying its control and function have not yet been well-defined. The nucleolar protein RSL24D1 was originally identified as a factor important for 60S ribosomal subunit biogenesis. In addition, the PeBoW (BOP1-PES1-WDR12) complex has been well-defined as required for pre-28S rRNA processing and cell proliferation. In this study, we show that RSL24D1 depletion impairs both pre-ribosomal RNA (pre-rRNA) transcription and mature 28S rRNA production, leading to decreased protein synthesis and p53 stabilization in human cells. Surprisingly, each of the PeBoW complex members is also required for pre-rRNA transcription. We demonstrate that RSL24D1 and WDR12 co-immunoprecipitate with the RNA polymerase I subunit, RPA194, and regulate its steady state levels. These results uncover the dual role of RSL24D1 and the PeBoW complex in multiple steps of ribosome biogenesis, and provide evidence implicating large ribosomal subunit biogenesis factors in pre-rRNA transcription control | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a 60S large ribosomal subunit | |
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| 650 | 4 | |a pre-rRNA processing | |
| 650 | 4 | |a rRNA transcription | |
| 650 | 4 | |a ribosome biogenesis | |
| 700 | 1 | |a Buhagiar, Amber F |e verfasserin |4 aut | |
| 700 | 1 | |a Bryant, Carson J |e verfasserin |4 aut | |
| 700 | 1 | |a Ogawa, Lisa M |e verfasserin |4 aut | |
| 700 | 1 | |a Abriola, Laura |e verfasserin |4 aut | |
| 700 | 1 | |a Surovtseva, Yulia V |e verfasserin |4 aut | |
| 700 | 1 | |a Baserga, Susan J |e verfasserin |4 aut | |
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