Proteomic identification and structural basis for the interaction between sorting nexin SNX17 and PDLIM family proteins
Copyright © 2022. Published by Elsevier Ltd..
The sorting nexin SNX17 controls endosomal recycling of transmembrane cargo proteins including integrins, the amyloid precursor protein, and lipoprotein receptors. This requires association with the Commander trafficking complex and depends on the C terminus of SNX17 through unknown mechanisms. Using proteomics, we find that the SNX17 C terminus is sufficient for Commander interaction and also associates with members of the PDZ and LIM domain (PDLIM) family. SNX17 contains a type III PDZ binding motif that binds specifically to the PDLIM proteins. The structure of the PDLIM7 PDZ domain bound to the SNX17 C terminus reveals an unconventional perpendicular peptide interaction mediated by electrostatic contacts and a uniquely conserved proline-containing loop sequence in the PDLIM protein family. Our results define the mechanism of SNX17-PDLIM interaction and suggest that the PDLIM proteins may play a role in regulating the activity of SNX17 in conjunction with Commander and actin-rich endosomal trafficking domains.
Errataetall: |
CommentIn: Structure. 2022 Dec 1;30(12):1559-1560. - PMID 36459972 |
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Medienart: |
E-Artikel |
Erscheinungsjahr: |
2022 |
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Erschienen: |
2022 |
Enthalten in: |
Zur Gesamtaufnahme - volume:30 |
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Enthalten in: |
Structure (London, England : 1993) - 30(2022), 12 vom: 01. Dez., Seite 1590-1602.e6 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Healy, Michael D [VerfasserIn] |
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Links: |
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Themen: |
Commander |
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Anmerkungen: |
Date Completed 06.12.2022 Date Revised 21.12.2022 published: Print-Electronic CommentIn: Structure. 2022 Dec 1;30(12):1559-1560. - PMID 36459972 Citation Status MEDLINE |
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doi: |
10.1016/j.str.2022.10.001 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM348167539 |
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500 | |a CommentIn: Structure. 2022 Dec 1;30(12):1559-1560. - PMID 36459972 | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Copyright © 2022. Published by Elsevier Ltd. | ||
520 | |a The sorting nexin SNX17 controls endosomal recycling of transmembrane cargo proteins including integrins, the amyloid precursor protein, and lipoprotein receptors. This requires association with the Commander trafficking complex and depends on the C terminus of SNX17 through unknown mechanisms. Using proteomics, we find that the SNX17 C terminus is sufficient for Commander interaction and also associates with members of the PDZ and LIM domain (PDLIM) family. SNX17 contains a type III PDZ binding motif that binds specifically to the PDLIM proteins. The structure of the PDLIM7 PDZ domain bound to the SNX17 C terminus reveals an unconventional perpendicular peptide interaction mediated by electrostatic contacts and a uniquely conserved proline-containing loop sequence in the PDLIM protein family. Our results define the mechanism of SNX17-PDLIM interaction and suggest that the PDLIM proteins may play a role in regulating the activity of SNX17 in conjunction with Commander and actin-rich endosomal trafficking domains | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 4 | |a Commander | |
650 | 4 | |a PDLIM | |
650 | 4 | |a PDZ domain | |
650 | 4 | |a SNX17 | |
650 | 4 | |a endosome | |
650 | 4 | |a retriever | |
650 | 7 | |a Sorting Nexins |2 NLM | |
700 | 1 | |a Sacharz, Joanna |e verfasserin |4 aut | |
700 | 1 | |a McNally, Kerrie E |e verfasserin |4 aut | |
700 | 1 | |a McConville, Calum |e verfasserin |4 aut | |
700 | 1 | |a Tillu, Vikas A |e verfasserin |4 aut | |
700 | 1 | |a Hall, Ryan J |e verfasserin |4 aut | |
700 | 1 | |a Chilton, Molly |e verfasserin |4 aut | |
700 | 1 | |a Cullen, Peter J |e verfasserin |4 aut | |
700 | 1 | |a Mobli, Mehdi |e verfasserin |4 aut | |
700 | 1 | |a Ghai, Rajesh |e verfasserin |4 aut | |
700 | 1 | |a Stroud, David A |e verfasserin |4 aut | |
700 | 1 | |a Collins, Brett M |e verfasserin |4 aut | |
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