Pre-and Postoperative Circulating Tumour DNA in Patients With Gastrointestinal Stromal Tumour - A Methodological Assessment Study

Copyright © 2022 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved..

BACKGROUND/AIM: Gastrointestinal stromal tumours (GISTs) harbour genetic aberrations in receptor tyrosine kinase KIT (KIT) or platelet-derived growth factor receptor A (PDGFRA) in 85-90% of the patients. Circulating tumour DNA (ctDNA) is a potential biomarker in patients with GIST. Previous studies investigating ctDNA around surgery in patients with GIST presented divergent results regarding the proportion of patients with detectable ctDNA. This study aimed to 1) investigate the feasibility of detecting and monitoring ctDNA pre-and postoperative, 2) compare two different circulating free DNA (cfDNA) extraction methods, and validate results obtained by next-generation sequencing (NGS) using Real-Time PCR technology.

PATIENTS AND METHODS: Eight patients planned for immediate surgery or surgery after neoadjuvant oncological treatment were included in the study, from whom blood collection was performed pre- and postoperatively for ctDNA analysis. Furthermore, blood samples from six patients with GIST harbouring a point mutation in KIT or PDGFRA in tissues from primary tumours were used for comparison and validation sub-study.

RESULTS: In this explorative study, none of the patients with very low to intermediate risk GIST harboured KIT, or PDGFRA mutated ctDNA in pre-or postoperative blood samples. The methods used for cfDNA extraction gave similar output, and the two methods for ctDNA analysis gave identical results.

CONCLUSION: There is no benefit in analysing ctDNA around surgery in very low to intermediate-risk GIST patients. Larger studies investigating ctDNA in patients with high-risk GIST around surgery are warranted.

Medienart:

E-Artikel

Erscheinungsjahr:

2022

Erschienen:

2022

Enthalten in:

Zur Gesamtaufnahme - volume:42

Enthalten in:

Anticancer research - 42(2022), 11 vom: 26. Nov., Seite 5527-5537

Sprache:

Englisch

Beteiligte Personen:

Brinch, Charlotte Margareta [VerfasserIn]
Aggerholm-Pedersen, Ninna [VerfasserIn]
Poulsen, Tim Svenstrup [VerfasserIn]
Skovrider-Ruminski, Wojciech [VerfasserIn]
DE Heer, Pieter [VerfasserIn]
Penninga, Luit [VerfasserIn]
Krarup-Hansen, Anders [VerfasserIn]
Hogdall, Estrid [VerfasserIn]

Links:

Volltext

Themen:

Cell-Free Nucleic Acids
Circulating Tumor DNA
Circulating tumour DNA
EC 2.7.10.1
Gastrointestinal stromal tumour
Journal Article
Next-generation sequencing
Protein-Tyrosine Kinases
Proto-Oncogene Proteins c-kit
Receptor, Platelet-Derived Growth Factor alpha
Receptors, Platelet-Derived Growth Factor

Anmerkungen:

Date Completed 28.10.2022

Date Revised 28.10.2022

published: Print

Citation Status MEDLINE

doi:

10.21873/anticanres.16022

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM348032919