CDON contributes to Hedgehog-dependent patterning and growth of the developing limb
Copyright © 2022. Published by Elsevier Inc..
Hedgehog (HH) signaling is a major driver of tissue patterning during embryonic development through the regulation of a multitude of cell behaviors including cell fate specification, proliferation, migration, and survival. HH ligands signal through the canonical receptor PTCH1 and three co-receptors, GAS1, CDON and BOC. While previous studies demonstrated an overlapping and collective requirement for these co-receptors in early HH-dependent processes, the early embryonic lethality of Gas1;Cdon;Boc mutants precluded an assessment of their collective contribution to later HH-dependent signaling events. Specifically, a collective role for these co-receptors during limb development has yet to be explored. Here, we investigate the combined contribution of these co-receptors to digit specification, limb patterning and long bone growth through limb-specific conditional deletion of Cdon in a Gas1;Boc null background. Combined deletion of Gas1, Cdon and Boc in the limb results in digit loss as well as defects in limb outgrowth and long bone patterning. Taken together, these data demonstrate that GAS1, CDON and BOC are collectively required for HH-dependent patterning and growth of the developing limb.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:493 |
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| Enthalten in: |
Developmental biology - 493(2023) vom: 10. Jan., Seite 1-11 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Echevarría-Andino, Martha L [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 12.12.2022 Date Revised 13.04.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.ydbio.2022.09.011 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM347803369 |
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| 245 | 1 | 0 | |a CDON contributes to Hedgehog-dependent patterning and growth of the developing limb |
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| 500 | |a Date Revised 13.04.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2022. Published by Elsevier Inc. | ||
| 520 | |a Hedgehog (HH) signaling is a major driver of tissue patterning during embryonic development through the regulation of a multitude of cell behaviors including cell fate specification, proliferation, migration, and survival. HH ligands signal through the canonical receptor PTCH1 and three co-receptors, GAS1, CDON and BOC. While previous studies demonstrated an overlapping and collective requirement for these co-receptors in early HH-dependent processes, the early embryonic lethality of Gas1;Cdon;Boc mutants precluded an assessment of their collective contribution to later HH-dependent signaling events. Specifically, a collective role for these co-receptors during limb development has yet to be explored. Here, we investigate the combined contribution of these co-receptors to digit specification, limb patterning and long bone growth through limb-specific conditional deletion of Cdon in a Gas1;Boc null background. Combined deletion of Gas1, Cdon and Boc in the limb results in digit loss as well as defects in limb outgrowth and long bone patterning. Taken together, these data demonstrate that GAS1, CDON and BOC are collectively required for HH-dependent patterning and growth of the developing limb | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a BOC | |
| 650 | 4 | |a CDON | |
| 650 | 4 | |a Digit specification | |
| 650 | 4 | |a GAS1 | |
| 650 | 4 | |a HH co-receptors | |
| 650 | 4 | |a Hedgehog | |
| 650 | 4 | |a Limb development | |
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| 700 | 1 | |a Franks, Nicole E |e verfasserin |4 aut | |
| 700 | 1 | |a Schrader, Hannah E |e verfasserin |4 aut | |
| 700 | 1 | |a Hong, Mingi |e verfasserin |4 aut | |
| 700 | 1 | |a Krauss, Robert S |e verfasserin |4 aut | |
| 700 | 1 | |a Allen, Benjamin L |e verfasserin |4 aut | |
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